Department of Respiratory Medicine, Nara Medical University, Kashihara, Nara, Japan.
Major of Medical Life Systems, Graduate School of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, Japan.
Int J Chron Obstruct Pulmon Dis. 2023 Apr 19;18:615-624. doi: 10.2147/COPD.S385780. eCollection 2023.
Oxidative stress is an important mechanism for the development and progression of chronic obstructive pulmonary disease (COPD). It may also contribute to systemic manifestation in patients with COPD. Reactive oxygen species (ROS) including free radicals play a crucial role in oxidative stress in COPD. The aims of this study were to determine serum scavenging capacity profile against multiple free radicals and to evaluate its correlation with pathophysiology, exacerbations, and prognosis in patients with COPD.
Serum scavenging capacity profile against multiple free radicals comprising hydroxyl radical (OH), superoxide radical (O ), alkoxy radical (RO), methyl radical (CH), alkylperoxyl radical (ROO), and singlet oxygen (O) was assessed using the multiple free-radical scavenging method in 37 patients with COPD (mean age, 71 years; mean forced expiratory volume in 1 s, 55.2% predicted). The severity of emphysema was evaluated by Goddard classification on chest computed tomography. Exacerbations were recorded prospectively for 1 year and the overall mortality was assessed 5 years after the initial assessment.
OH scavenging capacity was significantly decreased (p < 0.05) and O and CH scavenging capacity tended to decrease in patients with COPD compared to that in healthy controls. On the other hand, ROO scavenging capacity tended to increase. In addition, RO scavenging capacity was associated with severity of emphysema (p < 0.05) and exacerbation frequency (p < 0.02). There was a difference in the profile of the scavenging capacity between survived and deceased patients with COPD for 5 years after initial assessment.
Characteristic profile of free radical scavenging capacity can provide insight into the pathophysiology and prognosis of patients with COPD.
氧化应激是慢性阻塞性肺疾病（COPD）发展和进展的重要机制。它也可能导致 COPD 患者出现全身表现。活性氧（ROS）包括自由基在 COPD 的氧化应激中起着至关重要的作用。本研究旨在确定对多种自由基的血清清除能力谱，并评估其与 COPD 患者的病理生理学、加重和预后的相关性。
使用多自由基清除法评估 37 例 COPD 患者（平均年龄 71 岁；平均 1 秒用力呼气量占预计值的 55.2%）的血清对多种自由基（包括羟自由基（OH）、超氧自由基（O ）、烷氧基自由基（RO）、甲基自由基（CH）、烷过氧自由基（ROO）和单线态氧（O ）的清除能力谱。肺气肿的严重程度通过胸部计算机断层扫描的 Goddard 分类进行评估。前瞻性记录了 1 年内的加重情况，并在初次评估后的 5 年内评估了总死亡率。
与健康对照组相比，COPD 患者的 OH 清除能力显著降低（p < 0.05），O 和 CH 清除能力也有降低趋势。另一方面，ROO 清除能力有升高趋势。此外，RO 清除能力与肺气肿的严重程度（p < 0.05）和加重频率（p < 0.02）相关。在初次评估后 5 年内，存活和死亡的 COPD 患者的清除能力谱存在差异。
自由基清除能力的特征谱可以深入了解 COPD 患者的病理生理学和预后。
