Hubei Engineering Technology Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou, Hubei 434025, People's Republic of China.
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
J Agric Food Chem. 2023 May 17;71(19):7221-7229. doi: 10.1021/acs.jafc.3c00782. Epub 2023 May 9.
The sweet potato whitefly, , (Gennadius) (Hemiptera:Aleyrodidae) is a global pest of crops. Neonicotinoids are efficient insecticides used for control of this pest. Insecticidal targets of neonicotinoids are insect nicotinic acetylcholine receptors (nAChRs). Here, we characterized and cloned the full length of the nAChR β1 subunit () in and confirmed the consistency of in MEAM1 and MED. Expression levels of in different developmental stages and body parts of adults were investigated and compared in MED. dsRNA was prepared to knock down in adult and significantly decreases the susceptibility to five neonicotinoid insecticides, including imidacloprid, clothianidin, thiacloprid, nitenpyram, and dinotefuran. This study indicated as a notable site influencing the susceptibility of to neonicotinoids.
甘薯粉虱，（Gennadius）（半翅目：粉虱科）是一种全球性的作物害虫。新烟碱类杀虫剂是用于防治这种害虫的有效杀虫剂。新烟碱类杀虫剂的杀虫靶标是昆虫烟碱型乙酰胆碱受体（nAChR）。在这里，我们在甘薯粉虱中鉴定并克隆了全长的 nAChRβ1 亚基（），并在 MEAM1 和 MED 中证实了在甘薯粉虱中的一致性。研究了不同发育阶段和成虫身体部位中 的表达水平，并在 MED 中进行了比较。制备了 dsRNA 以敲低成虫中的 ，并显著降低了对五种新烟碱类杀虫剂（包括吡虫啉、噻虫啉、噻虫嗪、吡虫威和呋虫胺）的敏感性。这项研究表明 是影响甘薯粉虱对新烟碱类杀虫剂敏感性的一个显著位点。
