Wildlife Epidemiology and Molecular Microbiology Laboratory (One Health Research Group), Discipline of Zoology, Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Ravi Campus, Pattoki, Pakistan; Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Ravi Campus, Pattoki, Pakistan.
Wildlife Epidemiology and Molecular Microbiology Laboratory (One Health Research Group), Discipline of Zoology, Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Ravi Campus, Pattoki, Pakistan; Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Ravi Campus, Pattoki, Pakistan.
Acta Trop. 2023 Jul;243:106940. doi: 10.1016/j.actatropica.2023.106940. Epub 2023 May 7.
Bartonella can infect a variety of mammals including humans and has been detected in the Americas, Europe, Africa, and Asia. Roughly two-thirds of identified Bartonella species are found and maintained in rodent reservoirs, with some of these species linked to human infections. Rodents (N=236) were caught from the Sahiwal division of Punjab, Pakistan and tested for Bartonella using PCR targeting gltA and rpoB genes, followed by sequencing of rpoB-positive samples. Genetic relatedness to other published Bartonella spp. rpoB gene sequences were examined using BLAST and phylogenetic analysis. Overall, 7.62% (18/236) of rodents were positive for both gltA and rpoB fragments. Rattus rattus and R. norvegicus had 7.94% (12/151) and 7.05% (6/85) positivity rates for Bartonella DNA, respectively. Phylogenetic analysis revealed a close relatedness between Bartonella spp. from Pakistan to Bartonella spp. from China, Nepal, and Malaysia. This study is the first reported detection of Bartonella spp. in R. rattus and R. norvegicus from the Sahiwal area of Punjab, Pakistan.
巴尔通体可以感染包括人类在内的多种哺乳动物，已在美洲、欧洲、非洲和亚洲被发现。大约三分之二已鉴定的巴尔通体物种存在于啮齿动物的储库中，并维持着这些物种的存在，其中一些物种与人类感染有关。从巴基斯坦旁遮普的萨希瓦尔地区捕获了 236 只啮齿动物，并使用针对 gltA 和 rpoB 基因的 PCR 对其进行了巴尔通体检测，随后对 rpoB 阳性样本进行了测序。使用 BLAST 和系统发育分析检查了与其他已发表的巴尔通体 spp. rpoB 基因序列的遗传相关性。总的来说，236 只啮齿动物中有 7.62%（18/236）对 gltA 和 rpoB 片段均呈阳性。褐家鼠和挪威鼠的巴尔通体 DNA 阳性率分别为 7.94%（12/151）和 7.05%（6/85）。系统发育分析显示，巴基斯坦的巴尔通体与来自中国、尼泊尔和马来西亚的巴尔通体密切相关。本研究首次报道了在巴基斯坦旁遮普的萨希瓦尔地区的褐家鼠和挪威鼠中检测到巴尔通体。
