Division of Digestive Diseases, University of Cincinnati College of Medicine, Ohio.
Eurofins Viracor Laboratories, Lenexa, Kansas.
J Infect Dis. 2023 Nov 2;228(9):1208-1218. doi: 10.1093/infdis/jiad117.
BK polyomavirus (BKPyV) infection is common after hematopoietic stem cell transplantation (HSCT) and is associated with the development of hemorrhagic cystitis (HC). The role that BKPyV plays in the pathogenesis of HC is not well characterized. We investigated the impact of BKPyV diversity on the development of HC using a previously established cohort of pediatric HSCT patients. There were 147 urine samples with quantifiable BKPyV at month 1 after HSCT; 137 (93.2%) were amplified using our in-house polymerase chain reaction approach and sent for next-generation sequencing. Subtype Ia was most frequent (61.3%), followed by subtype Ib1 (31.4%). The median viral load of subtype Ia samples was higher than for subtype Ib1 at month 1. Across the protein coding regions, APOBEC-induced mutations and signature patterns associated with HC were identified. This is the largest sequencing study of a single cohort of HSCT patients, providing a vast resource of sequence data for future analyses.
BK 多瘤病毒 (BKPyV) 感染在造血干细胞移植 (HSCT) 后很常见，并且与出血性膀胱炎 (HC) 的发展有关。BKPyV 在 HC 发病机制中的作用尚未很好地描述。我们使用先前建立的儿科 HSCT 患者队列研究了 BKPyV 多样性对 HC 发展的影响。在 HSCT 后 1 个月有 147 份可定量 BKPyV 的尿液样本；137 份（93.2%）使用我们的内部聚合酶链反应方法进行了扩增，并进行了下一代测序。亚型 Ia 最为常见（61.3%），其次是 Ib1 亚型（31.4%）。在 1 个月时，Ia 型样本的病毒载量中位数高于 Ib1 型。在整个蛋白编码区中，鉴定了 APOBEC 诱导的突变和与 HC 相关的特征模式。这是对单个 HSCT 患者队列的最大测序研究，为未来的分析提供了大量的序列数据资源。
