School of Biosciences and Bioengineering, Indian Institute of Technology Mandi, Kamand, Mandi, Himachal Pradesh 175005, India.
School of Biological Sciences, National Institute of Science Education and Research, HBNI, Jatni, Odisha 752050, India.
ACS Appl Mater Interfaces. 2023 May 24;15(20):24209-24227. doi: 10.1021/acsami.3c04390. Epub 2023 May 11.
Rapid emergence of multidrug-resistant bacterial strains has posed a global threat to public health. Hospital-acquired infections, especially in diabetic and burn patients, severely impede the process of wound healing, thereby causing high mortality. This calls for developing a new biomaterial that synergistically destroys pathogenic strains and also helps in promoting wound healing without causing any resistance generation. A new and highly potent antibacterial agent has been developed by integrating the bactericidal and wound healing properties of MoS nanosheets and a recently developed quaternized polysaccharide, pullulan (CP), into a single nanoplatform for accelerated wound therapy. MoS nanosheets are noncovalently functionalized with quaternized pullulan to yield glycosheets (MCP) that efficiently eradicate both Gram-negative (5 μg/mL) and Gram-positive (10 μg/mL) within a short period of 4 h, through a synergistic action of membrane damage and chemical oxidation. MoS nanosheets coupled with CP exert a membrane-directed bactericidal action through distinct mechanisms of "pore-forming" and "non-pore-forming" pathways, respectively, whereas oxidative stress is induced by MoS nanosheets alone to collectively kill the pathogens. The MCP glycosheets have good biocompatibility and are also capable of disrupting and eradicating mature biofilms. Rapid and highly efficient wound disinfection and healing occurred upon MCP treatment through the reduction of inflammation and promotion of cellular proliferation and tissue remodeling. Thus, MCP glycosheets can emerge as a safe and potential biomaterial for better wound care management.
耐药细菌株的快速出现对全球公共卫生构成了威胁。医院获得性感染，特别是在糖尿病和烧伤患者中，严重阻碍了伤口愈合过程，从而导致高死亡率。因此，需要开发一种新的生物材料，该材料既能协同破坏致病菌，又能促进伤口愈合，同时不会产生任何耐药性。通过将 MoS 纳米片的杀菌和伤口愈合特性与最近开发的季铵化多糖普鲁兰（CP）集成到单个纳米平台中，开发出了一种新型高效抗菌剂，用于加速伤口治疗。MoS 纳米片通过非共价键功能化季铵化普鲁兰，得到糖基片（MCP），在 4 小时内通过膜损伤和化学氧化的协同作用，有效地消灭革兰氏阴性菌（5 μg/mL）和革兰氏阳性菌（10 μg/mL）。MoS 纳米片与 CP 共同作用，通过“形成孔”和“非形成孔”途径的不同机制发挥膜定向杀菌作用，而氧化应激则由 MoS 纳米片单独诱导，从而共同杀死病原体。MCP 糖基片具有良好的生物相容性，也能够破坏和根除成熟的生物膜。通过减少炎症、促进细胞增殖和组织重塑，MCP 处理后伤口迅速高效地消毒和愈合。因此，MCP 糖基片有望成为一种安全有效的生物材料，用于更好的伤口护理管理。
