拟南芥微嫁接研究营养状况的系统信号。
Arabidopsis Micro-grafting to Study the Systemic Signaling of Nutrient Status.
机构信息
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.
出版信息
Methods Mol Biol. 2023;2665:113-120. doi: 10.1007/978-1-0716-3183-6_10.
Grafting enables the study of systemic signals that plants use to maintain their homeostasis at the level of the whole organism. Several protocols of Arabidopsis grafting have been published over the years. These methods are limited because they either affect the overall behavior of the plant, or their throughput is low. The method presented here is based on grafting 3- to 4-days-old seedlings directly on an agar plate, without the use of hormone or collar, and can produce consistently over a hundred grafted plants per day and operator.
嫁接使研究植物在整个生物体水平上维持其体内平衡所使用的系统信号成为可能。多年来已经发表了几种拟南芥嫁接的方案。这些方法受到限制，因为它们要么影响植物的整体行为，要么其通量低。本文介绍的方法是将 3 至 4 天大的幼苗直接在琼脂板上进行嫁接，不使用激素或环，每天和每个操作人员可以稳定地生产超过一百株嫁接植物。
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