大鼠骨骼肌中通过线粒体外苹果酸酶进行丙酮酸的羧化作用。

Carboxylation of pyruvate via extramitochondrial malic enzyme in rat skeletal muscle.

作者信息

Swierczyński J, Aleksandrowicz Z, Zelewski L

出版信息

Acta Biochim Pol. 1986;33(1):47-54.

Abstract

Soluble rat skeletal muscle extract incubated in the presence of pyruvate, NaH14CO3, MgCl2 and NADP-generating system fixed H14CO3- at the rate of about 2.3 nmol/min per mg protein. The omission of pyruvate or NADPH-generating system abolished completely the fixation of H14CO3-. This suggests that malic enzyme is responsible for the carboxylation of pyruvate. A possible role of pyruvate carboxylation by extramitochondrial malic enzyme in the conversion of lactate into glycogen in skeletal muscle is discussed.

摘要

在丙酮酸、NaH¹⁴CO₃、MgCl₂和NADP生成系统存在的情况下孵育的可溶性大鼠骨骼肌提取物，以约2.3 nmol/（min·mg蛋白质）的速率固定H¹⁴CO₃⁻。省略丙酮酸或NADPH生成系统会完全消除H¹⁴CO₃⁻的固定。这表明苹果酸酶负责丙酮酸的羧化作用。讨论了线粒体外苹果酸酶催化的丙酮酸羧化作用在骨骼肌中将乳酸转化为糖原过程中的可能作用。