Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
Administration of Agriculture and Rural Affairs of Hongze District, Huai'an City, Huai'an, 223199, China.
Plant Physiol Biochem. 2023 Jun;199:107726. doi: 10.1016/j.plaphy.2023.107726. Epub 2023 Apr 28.
Triterpenoids, known for their anti-inflammatory, anticancer, and hypoglycemic properties, are the major bioactive components in Cyclocarya paliurus (Batal.) Iljinskaja. Selecting elite individuals with high triterpenoids content is the basis of C. paliurus industry for medicinal use. In this study, seasonal variation patterns of total triterpenoids and five triterpene monomers accumulation for three groups with different total triterpenoid contents (TTC; H: 59.74-64.03 mg g; M: 47.66-57.08 mg g, and L: 35.26-42.22 mg g) were surveyed. Seasonal expression dynamics of 6 key genes relevant to triterpenoids biosynthesis, including HMGR, DXR, SQS, SE, LUS, and β-AS, were described by quantitative real-time PCR (qRT-PCR) for three groups. The expression levels of HMGR, SE, LUS, and β-AS genes in group H were higher than in groups M and L. In addition, Pearson correlation analysis showed that they were significantly positively correlated with triterpene accumulation, and the expression level of SE gene not only was significantly correlated with downstream genes, but also exhibited a linear relationship with TTC, especially in September. These results suggest that SE gene could serve as an effective make for screening elite individuals with high TTC from the germplasm of C. paliurus for medicinal use. Further testing on randomly selected individuals in next September proved the feasibility and reliability of SE gene in assisted selection. Also, we successfully cloned the full-length cDNA of SE. Thus, our work provides an efficient way to attain superior genotypes to develop medicinal industry of C. paliurus in practice.
三萜类化合物具有抗炎、抗癌和降血糖作用，是青钱柳叶（Cyclocarya paliurus (Batal.) Iljinskaja）中的主要生物活性成分。选择总三萜含量高的优良个体是青钱柳药用产业的基础。本研究调查了三组（H 组：59.74-64.03mg/g；M 组：47.66-57.08mg/g，和 L 组：35.26-42.22mg/g）不同总三萜含量的季节性总三萜和五种三萜单体积累的变化模式。通过定量实时 PCR（qRT-PCR）描述了与三萜生物合成相关的 6 个关键基因（HMGR、DXR、SQS、SE、LUS 和 β-AS）在三组中的季节性表达动态。H 组的 HMGR、SE、LUS 和 β-AS 基因表达水平高于 M 组和 L 组。此外，Pearson 相关分析表明，它们与三萜积累呈显著正相关，SE 基因的表达水平不仅与下游基因显著相关，而且与 TTC 呈线性关系，尤其是在 9 月。这些结果表明，SE 基因可作为一种有效的方法，从青钱柳的种质资源中筛选出总三萜含量高的优良个体，用于药用。明年 9 月对随机选择的个体进行进一步测试证明了 SE 基因在辅助选择中的可行性和可靠性。此外，我们成功克隆了 SE 的全长 cDNA。因此，我们的工作为开发青钱柳药用产业提供了一种有效的方法，可以获得优良的基因型。
