Patt H M, Maloney M A
Blood Cells. 1978;4(1-2):27-35.
In studies with bgJ/bgJ:W/WV chimeric mice, we used the biege neutrophil marker as a criterion of of W/WV marrow replacement by implanted bgJ/bg/J stem cells. Data from a 50-fold range of inoculum doses and a 2-year period of observation indicate a hyperbolic pattern of replacement expressed as a log dose-response relationship. The saturating effect with increasing inoculum dose was interpreted as reflecting random initial stem cell seeding in bone marrow coupled with a decreasing efficiency of colonization by migration. From the statistics of random sampling and the exponential decrease of the 63% replacement dose with time, we estimate that W/WV marrow contains about 2600 stem cell regulatory volumes of about 10(8) mu3 (50 cell diameters) each, a dimension consistent with concepts of short-range cell-cell interactions. Our observations suggest that each regulatory volume is essentially self-contained and that stem cell migration is generally restricted to contiguous volumes.
在对bgJ/bgJ:W/WV嵌合小鼠的研究中，我们使用米色中性粒细胞标记物作为植入的bgJ/bg/J干细胞替代W/WV骨髓的标准。来自50倍接种剂量范围和2年观察期的数据表明，替代呈现双曲线模式，表现为对数剂量反应关系。接种剂量增加时的饱和效应被解释为反映了骨髓中随机的初始干细胞播种以及迁移导致的定植效率降低。根据随机抽样统计和63%替代剂量随时间的指数下降，我们估计W/WV骨髓含有约2600个干细胞调节单位，每个调节单位约为10(8)立方微米（50个细胞直径），这一尺寸与短程细胞间相互作用的概念一致。我们的观察结果表明，每个调节单位基本是自我包含的，并且干细胞迁移通常局限于相邻的单位。
