Sarantonis E G, Diamandis E P, Karayannis M I
Anal Biochem. 1986 May 15;155(1):129-34. doi: 10.1016/0003-2697(86)90237-x.
Potentiometric studies of the reaction between trinitrobenzenesulfonic acid (TNBS) and several amino acids with the TNBS electrode indicate that the reaction is first-order with respect to TNBS and amino acid concentration. The reaction is zero-order with respect to hydroxide concentration at pH greater than 10.5, indicating that the nonprotonated amino group is the reactive species. Rate constants were calculated for each amino acid and a simple mechanism of the reaction is proposed.
