Couto Joana, Gonçalves Rute, Lamas Sofia, Saraiva Margarida
i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4200-135, Portugal.
i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4200-135, Portugal; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto 4200-135, Portugal.
STAR Protoc. 2023 Apr 27;4(2):102303. doi: 10.1016/j.xpro.2023.102303.
Intranasal infection of k18-hACE2 mice with SARS-CoV-2 recapitulates the clinical characteristics present in severe COVID-19. Here, we present a protocol for intranasal administration of SARS-CoV-2 to k18-hACE2 mice and their subsequent daily monitoring. We describe steps for intranasal inoculation of SARS-CoV-2 and the collection of clinical scores on weight, body condition, hydration, appearance, neurological symptoms, behavior, and respiratory movements. This protocol contributes to the establishment of a model of severe SARS-CoV-2 infection that minimizes animal suffering. For complete details on the use and execution of this protocol, please refer to Gonçalves et al. (2023)..
用SARS-CoV-2对k18-hACE2小鼠进行鼻内感染可重现重症COVID-19的临床特征。在此，我们介绍一种向k18-hACE2小鼠鼻内接种SARS-CoV-2并随后进行每日监测的方案。我们描述了鼻内接种SARS-CoV-2的步骤以及收集关于体重、身体状况、水合作用、外观、神经症状、行为和呼吸运动的临床评分的步骤。该方案有助于建立一个将动物痛苦降至最低的重症SARS-CoV-2感染模型。有关该方案的使用和实施的完整详细信息，请参考Gonçalves等人（2023年）的研究。
