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发光热变质人体遗骸的温度特异光谱位移。

Temperature-specific spectral shift of luminescing thermally altered human remains.

机构信息

Amsterdam UMC location University of Amsterdam, Biomedical Engineering and Physics, Amsterdam, The Netherlands.

Amsterdam UMC location University of Amsterdam, Medical Biology, Section Clinical Anatomy and Embryology, Amsterdam, The Netherlands.

出版信息

Int J Legal Med. 2023 Jul;137(4):1277-1286. doi: 10.1007/s00414-023-03006-0. Epub 2023 May 13.

DOI:10.1007/s00414-023-03006-0
PMID:37178277
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10247558/
Abstract

Human bone has shown to have luminescent properties that remain throughout the phases of cremation, with the exception of fully carbonized bone, when excited with a narrow band light source. During this research, an alternate light source (420-470nm, peak at 445nm) was used to visualize and investigate latent details relevant for forensic investigations of human remains recovered at fire scenes. As fire is a destructive force, it induces a vast variety of physical and chemical alterations to all components of the bone, making the subsequent analysis and interpretation of burned human remains challenging. A spectral shift in emission bandwidth, from green to red, was previously observed when the exposure temperature increased from 700 to 800 °C. This spectral shift was reproduced on a total of 10 human forearms, divided into 20 segments, by burning at 700 °C and 900 °C in an ashing furnace. The shift of emission bandwidth caused only by an increase in temperature was furthermore investigated by colorimetric analysis, proving the spectral shift to be significant. By easily quantifying the spectral shift, substantiation is provided for the use of this technique in practice to improve the interpretation of heat induced changes of bone.

摘要

人类骨骼已被证明具有发光特性,在火化的各个阶段都存在,除了完全碳化的骨骼,当用窄带光源激发时。在这项研究中,使用了另一种光源(420-470nm,峰值在 445nm)来可视化和研究与在火灾现场发现的人类遗骸的法医学调查相关的潜在细节。由于火灾是一种破坏性力量,它会对骨骼的所有成分产生各种物理和化学变化,从而使随后对燃烧的人类遗骸进行分析和解释变得具有挑战性。以前观察到,当暴露温度从 700°C 增加到 800°C 时,发射带宽的光谱位移从绿色变为红色。通过在灰化炉中以 700°C 和 900°C 燃烧总共 10 个人类前臂,将其分成 20 个部分,重现了这种光谱位移。通过比色分析进一步研究了仅由温度升高引起的发射带宽位移,证明了光谱位移是显著的。通过轻松量化光谱位移,为在实践中使用该技术提供了依据,以改善对骨骼受热诱导变化的解释。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/d1227efec139/414_2023_3006_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/f358f0a27945/414_2023_3006_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/9c1de119cc3c/414_2023_3006_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/14ff00bcc490/414_2023_3006_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/1178f9d74013/414_2023_3006_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/0ad16223e2de/414_2023_3006_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/d1227efec139/414_2023_3006_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/f358f0a27945/414_2023_3006_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/9c1de119cc3c/414_2023_3006_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/14ff00bcc490/414_2023_3006_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/1178f9d74013/414_2023_3006_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/0ad16223e2de/414_2023_3006_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b6f/10247558/d1227efec139/414_2023_3006_Fig6_HTML.jpg

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Estimating temperature exposure of burnt bone - A methodological review.烧伤骨骼温度暴露的估算——方法学综述
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A potential new diagnostic tool to aid DNA analysis from heat compromised bone using colorimetry: A preliminary study.
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NIH Image to ImageJ: 25 years of image analysis.NIH 图像到 ImageJ:25 年的图像分析。
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