• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

流式细胞术自动化:系统指南和评价。

Automation in flow cytometry: Guidelines and review of systems.

机构信息

UMass Memorial Medical Center, UMass Chan Medical School, Worcester, Massachusetts, USA.

Medical City Dallas, Medical City Children's Hospital, Dallas, Texas, USA.

出版信息

Cytometry B Clin Cytom. 2024 Jul;106(4):308-320. doi: 10.1002/cyto.b.22125. Epub 2023 May 13.

DOI:10.1002/cyto.b.22125
PMID:37178358
Abstract

Automation in flow cytometry has recently advanced from the partial laboratory automation and robotics islets, to more fully integrated systems. This article reviews three manufacturers' newest sample preparation systems: the Beckman CellMek, the Sysmex PS-10, and the BD FACSDuet. These three instruments are capable of performing many of the manual steps in flow cytometry sample processing (pipetting, staining, lysing, washing, fixing). General description, capabilities, advantages, and disadvantages of each system are compared. Overall, these systems have the potential to become mainstay items in today's busy clinical flow cytometry laboratories, and save a significant amount of hands-on time for laboratory staff.

摘要

流式细胞仪的自动化技术最近已从部分实验室自动化和机器人小岛发展到更加完全集成的系统。本文综述了三家制造商的最新样本制备系统:贝克曼库尔特公司的 CellMek、希森美康公司的 PS-10 和 BD 公司的 FACSDuet。这三种仪器能够执行流式细胞术样本处理中的许多手动步骤(移液、染色、裂解、洗涤、固定)。对每个系统的一般描述、功能、优点和缺点进行了比较。总的来说,这些系统有可能成为当今繁忙的临床流式细胞术实验室的主要项目,并为实验室工作人员节省大量的实际操作时间。

相似文献

1
Automation in flow cytometry: Guidelines and review of systems.流式细胞术自动化:系统指南和评价。
Cytometry B Clin Cytom. 2024 Jul;106(4):308-320. doi: 10.1002/cyto.b.22125. Epub 2023 May 13.
2
Impact of the new Beckman Coulter Cytomics FC 500 5-color flow cytometer on a regional flow cytometry clinical laboratory service.新型贝克曼库尔特Cytomics FC 500五参数流式细胞仪对某地区流式细胞术临床实验室服务的影响
Lab Hematol. 2004;10(2):102-8. doi: 10.1532/LH96.04121.
3
Automation in Flow Cytometry.流式细胞术自动化。
Clin Lab Med. 2024 Sep;44(3):455-463. doi: 10.1016/j.cll.2024.04.007. Epub 2024 May 30.
4
Standardization of 8-color flow cytometry across different flow cytometer instruments: A feasibility study in clinical laboratories in Switzerland.8 色流式细胞仪在不同流式细胞仪仪器上的标准化:瑞士临床实验室的可行性研究。
J Immunol Methods. 2019 Dec;475:112348. doi: 10.1016/j.jim.2017.07.013. Epub 2017 Jul 29.
5
Streamlining basophil activation testing to enable assay miniaturization and automation of sample preparation.简化嗜碱性粒细胞活化试验,实现检测微量化和样本制备自动化。
J Immunol Methods. 2020 Jun-Jul;481-482:112793. doi: 10.1016/j.jim.2020.112793. Epub 2020 May 6.
6
Evaluation of a novel automated volumetric flow cytometer for absolute CD4+ T lymphocyte quantitation.评价一种新型自动化全容积流式细胞仪用于绝对 CD4+T 淋巴细胞定量检测。
Cytometry B Clin Cytom. 2017 Nov;92(6):456-464. doi: 10.1002/cyto.b.21360. Epub 2016 Feb 23.
7
Workflow improvement and financial gain after integration of high-throughput sample processing system with flow cytometer in a high-volume pathology laboratory: Results from a prospective comparative study using Lean principles.高通量样本处理系统与流式细胞仪在大容量病理实验室整合后的工作流程改进和经济效益:应用精益原则的前瞻性对照研究结果。
J Immunol Methods. 2024 Nov;534:113767. doi: 10.1016/j.jim.2024.113767. Epub 2024 Oct 18.
8
Polychromatic flow cytometry in the clinical laboratory.临床实验室中的多色流式细胞术。
Pathology. 2011 Oct;43(6):580-91. doi: 10.1097/PAT.0b013e32834a69ae.
9
Automation for clinical CD4 T-cell enumeration, a desirable tool in the hands of skilled operators.临床 CD4 T 细胞计数的自动化,对于熟练操作人员来说是一个理想的工具。
Cytometry B Clin Cytom. 2017 Nov;92(6):445-450. doi: 10.1002/cyto.b.21370. Epub 2016 Mar 30.
10
External quality assessment in clinical cell analysis by flow cytometry. Why is it so important?流式细胞术临床细胞分析中的外部质量评估。为何如此重要?
Coll Antropol. 2010 Mar;34(1):207-17.

引用本文的文献

1
An Update on Flow Cytometry Analysis of Hematological Malignancies: Focus on Standardization.血液系统恶性肿瘤流式细胞术分析的最新进展:聚焦标准化
Cancers (Basel). 2025 Jun 19;17(12):2045. doi: 10.3390/cancers17122045.
2
Cost-effective in-house-made whole blood materials for internal quality control in clinical flow cytometry analysis.用于临床流式细胞术分析内部质量控制的经济高效的自制全血材料。
Anal Bioanal Chem. 2025 Apr;417(10):2121-2132. doi: 10.1007/s00216-025-05801-7. Epub 2025 Feb 18.
3
Non-Microbiological Mycobacterial Detection Techniques for Quality Control of Biological Products: A Comprehensive Review.
生物制品质量控制中的非微生物分枝杆菌检测技术:综述
Microorganisms. 2024 Apr 12;12(4):788. doi: 10.3390/microorganisms12040788.