Asch B B, Medina D, Brinkley B R
Cancer Res. 1979 Mar;39(3):893-907.
The distribution and organization of microtubules (MT's) and actin-containing microfilaments (MF's) were examined in epithelial cells of primary cultures established from normal, preneoplastic, and neoplastic mouse mammary tissues and in cells of three clonal culture lines derived from murine mammary adenocarcinomas. No consistent differences in the cytoskeletal components were found among cell populations of the primary cultures as revealed either by indirect immunofluorescence using antibodies to tubulin and actin or by electron microscopy. Overall, the majority of cells in the three types of primary cultures possessed elaborate complexes of MT's and actin filaments after fluorescent staining with the appropriate antibodies, and abundant MT's and MF's were found in the cells at the ultrastructural level. Similar patterns of MT's and MF's were observed in cells of two of the clonal mammary tumor lines. Cells of the third line, however, exhibited intricate networks of MT's but had a reduction in actin cables detectable by the immunofluorescence procedure. Moreover, MF's were difficult to locate by electron microscopy. The results suggest that the lesion(s) in growth control in the neoplastic mammary cells may not involve any gross alterations in MT's or MF's.
在从正常、癌前和肿瘤性小鼠乳腺组织建立的原代培养上皮细胞以及源自小鼠乳腺腺癌的三个克隆培养系的细胞中，研究了微管（MT）和含肌动蛋白的微丝（MF）的分布与组织情况。通过使用抗微管蛋白和肌动蛋白抗体的间接免疫荧光法或电子显微镜检查发现，原代培养细胞群体之间在细胞骨架成分上未发现一致的差异。总体而言，在用适当抗体进行荧光染色后，三种原代培养类型中的大多数细胞都具有复杂的微管和肌动蛋白丝复合物，并且在超微结构水平上细胞中发现了丰富的微管和微丝。在两个克隆乳腺肿瘤系的细胞中观察到了类似的微管和微丝模式。然而，第三个系的细胞表现出复杂的微管网，但通过免疫荧光程序检测到肌动蛋白束减少。此外，通过电子显微镜很难定位微丝。结果表明，肿瘤性乳腺细胞生长控制中的病变可能不涉及微管或微丝的任何明显改变。
