Kütt Mary-Liis, Orgusaar Kaisa, Stulova Irina, Priidik Reimo, Pismennõi Dmitri, Vaikma Helen, Kallastu Aili, Zhogoleva Aleksandra, Morell Indrek, Kriščiunaite Tiina
Center of Food and Fermentation Technologies, Mäealuse 2/4, Tallinn, 12618, Estonia.
Institute of Chemistry and Biotechnology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia.
Heliyon. 2023 Apr 25;9(5):e15627. doi: 10.1016/j.heliyon.2023.e15627. eCollection 2023 May.
In the present study, an oat drink, a plant-based alternative to dairy products, was developed by fermenting the oat base with different vegan starter cultures. The desired pH below 4.2 was achieved in 12 h, regardless of starter culture used. Metagenomic sequencing revealed that was the dominating species, ranging from 38% to 99% of the total microbial consortia At lower pH values, population of , and continued to increase in fermented oat drinks. Lactic acid was produced between 1.6 and 2.8 g/L. The sensory panel showed that all fermented oat drinks had a sour odor and taste. The volatile compounds identified belonged to the ketone, alcohol, aldehyde, acids, and furan classes. The concentration of the most preferred volatile components, such as diacetyl and acetoin, increased during fermentation. However, sensory evaluation showed that all samples were associated with cereals and not dairy in terms of taste and odor. Rheological analysis showed the formation of weak gel-like structures in fermented oat drinks. Overall, fermentation improved flavor and texture of the product. This study provides a broad overview of the oat drink fermentation process from the perspectives of starter culture growth, microbial consortium dynamics, lactic acid bacteria metabolism, and sensory profile formation.
在本研究中,通过用不同的纯素发酵剂发酵燕麦基料,开发出了一种燕麦饮料,作为乳制品的植物性替代品。无论使用何种发酵剂,在12小时内均可达到低于4.2的理想pH值。宏基因组测序显示, 是优势菌种,占微生物群落总数的38%至99%。在较低的pH值下,发酵燕麦饮料中 、 和 的数量持续增加。乳酸产量在1.6至2.8克/升之间。感官评价小组表明,所有发酵燕麦饮料都有酸味和味道。鉴定出的挥发性化合物属于酮类、醇类、醛类、酸类和呋喃类。在发酵过程中,最受欢迎的挥发性成分如双乙酰和乙偶姻的浓度增加。然而,感官评价表明,就味道和气味而言,所有样品都与谷物有关,而非乳制品。流变学分析表明,发酵燕麦饮料中形成了弱凝胶状结构。总体而言,发酵改善了产品的风味和质地。本研究从发酵剂生长、微生物群落动态、乳酸菌代谢和感官特征形成等角度,对燕麦饮料发酵过程进行了全面概述。