Fuentes-Domínguez Rafael, Yao Mengting, Hardiman William, La Cavera Iii Salvatore, Setchfield Kerry, Pérez-Cota Fernando, Smith Richard J, Clark Matt
Optics and Photonics Group, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
Photoacoustics. 2023 Apr 24;31:100493. doi: 10.1016/j.pacs.2023.100493. eCollection 2023 Jun.
In this paper, we show a proof-of-concept method to parallelise phonon microscopy measurements for cell elasticity imaging by demonstrating a 3-fold increase in acquisition speed which is limited by current acquisition hardware. Phonon microscopy is based on time-resolved Brillouin scattering, which uses a pump-probe method with asynchronous optical sampling (ASOPS) to generate and detect coherent phonons. This enables access to the cell elasticity via the Brillouin frequency with sub-optical axial resolution. Although systems based on ASOPS are typically faster compared to the ones built with a mechanical delay line, they are still very slow to study real time changes at the cellular level. Additionally, the biocompatibility is reduced due to long light exposure and scanning time. Using a multi-core fibre bundle rather than a single channel for detection, we acquire 6 channels simultaneously allowing us to speed-up measurements, and open a way to scale-up this method.
在本文中，我们展示了一种用于细胞弹性成像的声子显微镜测量并行化的概念验证方法，通过证明采集速度提高了三倍，而这一速度受当前采集硬件的限制。声子显微镜基于时间分辨布里渊散射，它使用带有异步光学采样（ASOPS）的泵浦 - 探测方法来产生和检测相干声子。这使得能够通过布里渊频率以亚光学轴向分辨率获取细胞弹性信息。尽管基于ASOPS的系统通常比基于机械延迟线构建的系统速度更快，但在研究细胞水平的实时变化时仍然非常缓慢。此外，由于长时间的光暴露和扫描时间，生物相容性会降低。通过使用多芯光纤束而不是单通道进行检测，我们能够同时采集6个通道的数据，从而加快测量速度，并为扩大该方法的规模开辟了道路。
