Mohammed VI Polytechnic University, AgoBioSciences, Plant Stress Physiology Laboratory, Benguerir, 43150, Morocco; Department of Plant Production, Protection and Biotechnology, Hassan II Institute of Agronomy and Veterinary Medicine, Madinate Al Irfane, Morocco.
Department of Plant Production, Protection and Biotechnology, Hassan II Institute of Agronomy and Veterinary Medicine, Madinate Al Irfane, Morocco.
Plant Physiol Biochem. 2023 Jun;199:107718. doi: 10.1016/j.plaphy.2023.107718. Epub 2023 May 5.
Iron (Fe) deficiency is one of the most common problems of soybean. It causes upper leaves yellowing, interveinal chlorosis, stunted growth and yield loss. Manganese (Mn) deficiency affects the reactions in the oxygen evolving complex (OEC) of photosystem II and increase the accumulation of reactive oxygen species (ROS). The aim of this research is to study the effect of Fe and Mn deficiencies applied separately and simultaneously on physiological, biochemical, nutritional and growth (morphological) parameters of soybean cultivars (Glycine max L.). The experiment was conducted in nutrient hydroponic solution lacking Fe or Mn or both Fe and Mn. Chlorophyll content index (CCI) and chlorophyll a fluorescence were measured through time to detect nutritional disorders at an early growth stage before the apparition of visual symptoms. The results showed that Fe and Mn deficiencies had a significant negative effect on the photosynthetic efficiency, CCI, stomatal conductance, protein content and shoot/root nutrient uptakes. Iron and manganese stress conditions were found to enhance the accumulation of secondary metabolites and increase the antioxidant activity such as total polyphenol content (TPC), malondialdehyde (MDA) and superoxide dismutase (SOD). These impacts were more accentuated when Fe and Mn stress were applied simultaneously than when any of the deficiencies was applied alone. More than that, Mn stress alone did not significantly affect the biomass accumulation. The obtained results showed that, in hydroponic conditions, iron and manganese rational fertilization can improve the studied parameters.
铁（Fe）缺乏是大豆最常见的问题之一。它会导致上部叶片变黄、叶脉间失绿、生长受阻和产量损失。锰（Mn）缺乏会影响光系统 II 中的放氧复合体（OEC）的反应，并增加活性氧物质（ROS）的积累。本研究的目的是研究单独和同时施用铁和锰缺乏对大豆品种（Glycine max L.）的生理、生化、营养和生长（形态）参数的影响。该实验在缺乏铁或锰或同时缺乏铁和锰的营养水培溶液中进行。通过时间测量叶绿素含量指数（CCI）和叶绿素 a 荧光，以在出现视觉症状之前的早期生长阶段检测营养障碍。结果表明，铁和锰缺乏对光合作用效率、CCI、气孔导度、蛋白质含量和地上/地下养分吸收有显著的负面影响。发现铁和锰胁迫条件会增加次生代谢物的积累，并提高抗氧化活性，如总多酚含量（TPC）、丙二醛（MDA）和超氧化物歧化酶（SOD）。当同时施加铁和锰胁迫时，这些影响比单独施加任何一种缺乏时更为明显。此外，单独的锰胁迫不会显著影响生物量积累。所得结果表明，在水培条件下，铁和锰的合理施肥可以改善研究参数。
