State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China.
Chemosphere. 2023 Sep;334:138926. doi: 10.1016/j.chemosphere.2023.138926. Epub 2023 May 12.
In situ soils were collected at two depths in Jinan and Hangzhou steel plants, which both have a long history of operation and polycyclic aromatic hydrocarbons (PAHs) contamination. The richness of 16 S rRNA gene and bacterial community of the soil were determined by real-time PCR and high-throughput sequencing. Soil physicochemical properties, PAHs contamination characteristics, and their interrelationships were also analyzed. In general, the PAHs contamination decreased with increasing soil depths. The physicochemical properties and PAH concentration of soil had synergistic impacts on the composition of the bacterial community. The long-term higher PAHs stress in Hangzhou contaminated soil (982 mg kg) increased the bacterial abundance and diversity, while that of Jinan contaminated soil (63 mg kg) decreased bacterial abundance and diversity. The pH value, sand content of the soil were positively correlated (P < 0.05) with the bacterial diversity including Simpson, Shannon, Observed_species and Chao1 indexes., and the other soil properties exhibited negative correlations with different strengths. The abundances of Curvibacter, Pseudomonas, Thiobacillus, Lysobacter, and Limnobacter were positively correlated with the PAHs concentration (P < 0.01). Additionally, the network structure of the PAHs-contaminated soils was more complex compared to that of uncontaminated soils, with stronger linkages and correlations between the different bacteria. These findings provide a theoretical basis for microbial remediation of PAHs-polluted soil.
在济南和杭州的两家钢铁厂，采集了具有长期运行历史和多环芳烃（PAHs）污染的两个深度的原位土壤。通过实时 PCR 和高通量测序，确定了土壤中 16S rRNA 基因和细菌群落的丰富度。还分析了土壤理化性质、PAHs 污染特征及其相互关系。一般来说，随着土壤深度的增加，PAHs 污染程度降低。土壤理化性质和 PAH 浓度对细菌群落组成有协同影响。杭州污染土壤（982mg/kg）中存在长期较高的 PAHs 胁迫，增加了细菌丰度和多样性，而济南污染土壤（63mg/kg）则降低了细菌丰度和多样性。土壤 pH 值、沙含量与细菌多样性呈正相关（P<0.05），包括 Simpson、Shannon、Observed_species 和 Chao1 指数，而其他土壤性质则以不同的强度呈负相关。弯曲杆菌属、假单胞菌属、硫杆菌属、赖氨酸杆菌属和 Limnobacter 的丰度与 PAHs 浓度呈正相关（P<0.01）。此外，与未污染土壤相比，受 PAHs 污染土壤的网络结构更为复杂，不同细菌之间的联系和相关性更强。这些发现为 PAHs 污染土壤的微生物修复提供了理论依据。
