Hunan Provincial Key Lab of Dark Tea and Jin-hua, College of Chemistry and Material Engineering, Hunan City University, Yiyang, 413000, P. R. China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R.China.
Anal Methods. 2023 May 25;15(20):2505-2511. doi: 10.1039/d3ay00378g.
Copper plays a key role in the human body; meanwhile, excess Cu ions can result in various diseases. Nanoclusters (NCs) are often used to measure Cu ions, but there are two difficulties. On the one hand, a single probe of NCs is easily affected by environmental factors. On the other hand, it is difficult to mask the interference of Pb ions and Cd ions in the process of detecting Cu ions. As a new type of quantum dots (QDs), tungsten disulfide quantum dots (WS-QDs) have some advantages of simple synthesis and stable luminescence properties. Stable WS-QDs with blue fluorescence are used as a reference probe, while gold silver nanoclusters (AuAgNCs) with red fluorescence are used as a response probe. A ratiometric fluorescent sensor was constructed by mixing the two styles of fluorescent probes, which is abbreviated as NCs/QDs. This nano-sensor can be used to detect the concentration of Cu ions, in which the fluorescence of QDs does not change significantly, while the fluorescence of NCs can be quenched by Cu ions. The concentration of Cu ions can be determined as low as 0.12 μM with a linear range from 0.3 to 3 μM. The common interference caused by Pb and Cd ions can be eliminated by the phosphate buffer solution (PBS). This sensor was used to detect the concentration of Cu in river water with satisfactory results.
铜在人体中起着关键作用；同时，过量的 Cu 离子会导致各种疾病。纳米团簇（NCs）常用于测量 Cu 离子，但存在两个困难。一方面，NCs 的单一探针很容易受到环境因素的影响。另一方面，在检测 Cu 离子的过程中，很难屏蔽 Pb 离子和 Cd 离子的干扰。作为一种新型的量子点（QDs），二硫化钨量子点（WS-QDs）具有合成简单、发光性能稳定等优点。使用具有蓝色荧光的稳定 WS-QDs 作为参考探针，而具有红色荧光的金银纳米团簇（AuAgNCs）作为响应探针。将两种荧光探针混合构建比率荧光传感器，简称 NCs/QDs。该纳米传感器可用于检测 Cu 离子的浓度，其中 QDs 的荧光变化不明显，而 NCs 的荧光可以被 Cu 离子猝灭。Cu 离子的浓度可低至 0.12 μM 检测，线性范围为 0.3 至 3 μM。磷酸盐缓冲溶液（PBS）可以消除 Pb 和 Cd 离子引起的常见干扰。该传感器用于检测河水的 Cu 浓度，结果令人满意。
