Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Oral Dis. 2024 May;30(4):2051-2062. doi: 10.1111/odi.14613. Epub 2023 May 15.
Oral squamous cell carcinoma (OSCC) is the most common malignant tumour in the oral cavity. OSCC is aggressive and prone to metastasis; it is associated with high mortality and short survival. In this study, we investigated the function of the long non-coding RNA LINC00525 in OSCC progression and the molecular mechanisms through in vitro and in vivo experiments.
CCK8 assay was used to detect the effect of LINC00525 on cell viability; transwell migration and invasion assays and scratch assay were used to examine the role of LINC00525 in cell migration and invasion. Flow cytometry, RT-PCR and western blot were used to detect apoptosis indexes. Tumorigenic effects were investigated using mouse xenograft tumour models.
LINC00525 was associated with OSCC survival and prognosis. LINC00525 knockdown decreased cell viability and epithelial-mesenchymal transition (EMT) properties and increased apoptosis and also shortened the cell cycle of OSCC cells in vitro. The downregulation of LINC00525 reduced the growth of OSCC tumour in vivo. LINC00525 can regulate OSCC cells via the apoptotic signalling pathway.
Our results indicate that LINC00525 exhibits oncogenic functions in OSCC. LINC00525 may be a new promising and potential target for the treatment of OSCC.
口腔鳞状细胞癌（OSCC）是口腔中最常见的恶性肿瘤。OSCC 具有侵袭性和易转移性，死亡率高，存活时间短。本研究通过体外和体内实验，探讨长链非编码 RNA LINC00525 在 OSCC 进展中的功能及其分子机制。
采用 CCK8 法检测 LINC00525 对细胞活力的影响；Transwell 迁移和侵袭实验及划痕实验检测 LINC00525 对细胞迁移和侵袭的作用；流式细胞术、RT-PCR 和 Western blot 检测细胞凋亡指数。利用小鼠异种移植肿瘤模型研究肿瘤发生作用。
LINC00525 与 OSCC 患者的生存和预后相关。LINC00525 敲低降低了 OSCC 细胞的活力和上皮间质转化（EMT）特性，增加了细胞凋亡，并缩短了细胞周期。LINC00525 的下调减少了体内 OSCC 肿瘤的生长。LINC00525 通过凋亡信号通路调节 OSCC 细胞。
我们的研究结果表明，LINC00525 在 OSCC 中具有致癌功能。LINC00525 可能是治疗 OSCC 的一种新的有前途和潜在的靶点。
