Wang Jinhua, Cheng Chao, Zhao Changyu, Wang Lanjun
Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University;
Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University; College of Water Conservancy and Civil Engineering, Shandong Agricultural University.
J Vis Exp. 2023 Apr 28(194). doi: 10.3791/64741.
Neonicotinoids, a class of insecticides, are widely used because of their novel modes of action, high insecticidal activity, and strong root uptake. Imidacloprid, the most widely used insecticide worldwide, is a representative first-generation neonicotinoid and is used in pest control for crops, vegetables, and fruit trees. With such a broad application of imidacloprid, its residue in crops has attracted increasing scrutiny. In the present study, 15 wheat seedlings were placed in a culture medium containing 0.5 mg/L or 5 mg/L imidacloprid for hydroculture. The content of imidacloprid in the wheat roots and leaves was determined after 1 day, 2 days, and 3 days of hydroculture to explore the migration and distribution of imidacloprid in wheat. The results showed that imidacloprid was detected both in the roots and leaves of the wheat plant, and the content of imidacloprid in the roots was higher than that in the leaves. Furthermore, the imidacloprid concentration in the wheat increased with increasing exposure time. After 3 days of exposure, the roots and leaves of the wheat in the 0.5 mg/L treatment group contained 4.55 mg/kg ± 1.45 mg/kg and 1.30 mg/kg ± 0.08 mg/kg imidacloprid, respectively, while the roots and leaves of the 5 mg/L treatment group contained 42.5 mg/kg ± 0.62 mg/kg and 8.71 mg/kg ± 0.14 mg/kg imidacloprid, respectively. The results from the present study allow for a better understanding of pesticide residues in crops and provide a data reference for the environmental risk assessment of pesticides.
新烟碱类杀虫剂因其新颖的作用方式、高杀虫活性和强大的根部吸收能力而被广泛使用。吡虫啉是全球使用最广泛的杀虫剂,是第一代新烟碱类杀虫剂的代表,用于农作物、蔬菜和果树的害虫防治。随着吡虫啉如此广泛的应用,其在作物中的残留受到了越来越多的关注。在本研究中,将15株小麦幼苗置于含有0.5毫克/升或5毫克/升吡虫啉的培养基中进行水培。在水培1天、2天和3天后测定小麦根和叶中吡虫啉的含量,以探究吡虫啉在小麦中的迁移和分布。结果表明,在小麦植株的根和叶中均检测到了吡虫啉,且根中吡虫啉的含量高于叶中。此外,小麦中吡虫啉的浓度随着暴露时间的增加而增加。暴露3天后,0.5毫克/升处理组小麦的根和叶中吡虫啉含量分别为4.55毫克/千克±1.45毫克/千克和1.30毫克/千克±0.08毫克/千克,而5毫克/升处理组小麦的根和叶中吡虫啉含量分别为42.5毫克/千克±0.62毫克/千克和8.71毫克/千克±0.14毫克/千克。本研究结果有助于更好地了解作物中的农药残留,并为农药的环境风险评估提供数据参考。