Winter W T, Taylor M G, Stevens E S, Morris E R, Rees D A
Biochem Biophys Res Commun. 1986 May 29;137(1):87-93. doi: 10.1016/0006-291x(86)91179-4.
Dermatan sulfate in the solid state has been studied by 13C CP/MAS nmr and X-ray diffraction in order to establish the ring conformation of the L-iduronate moiety. The solid state nmr spectrum is similar to the solution spectrum obtained previously, indicating that a ring conformation at least approximating to 1C4 predominates in the solid state. X-ray powder diffraction data from the same sample indicate the presence of the 8-fold helix form previously observed by fiber diffraction, and interpreted in terms of a 4C1 ring form. A likely explanation of the results is that a distorted 1C4 L-iduronate ring conformation, not considered in the initial X-ray analysis, may emerge to provide a satisfactory interpretation of all available physical-chemical data.
为确定L-艾杜糖醛酸部分的环构象，已通过13C CP/MAS核磁共振和X射线衍射对固态硫酸皮肤素进行了研究。固态核磁共振谱与先前获得的溶液谱相似，表明在固态中至少近似于1C4的环构象占主导。来自同一样品的X射线粉末衍射数据表明存在先前通过纤维衍射观察到的8重螺旋形式，并根据4C1环形式进行了解释。这些结果的一个可能解释是，初始X射线分析中未考虑的扭曲1C4 L-艾杜糖醛酸环构象可能出现，以对所有可用的物理化学数据提供令人满意的解释。
