Haseler L J, Brooks W M, Irving M G, Bulliman B T, Kuchel P W, Doddrell D M
Biochem Int. 1986 Apr;12(4):613-8.
A simple multipulse sequence has been used to monitor creatine kinase kinetics in rat skeletal muscle in vivo. Using these procedures, the forward (ATP synthesis) and reverse fluxes (phosphocreatine synthesis) have been calculated to be 8.98 +/- 0.6 and 10.7 +/- 0.8 mumoles/g wet wt/s (n = 5) respectively. These results suggest that in resting skeletal muscle most of the gamma ATP observed in 31P NMR spectra is cytosolic and rapidly exchanging with phosphocreatine. The high flux rates reflect the high catalytic capacity of creatine kinase in skeletal muscle.
一种简单的多脉冲序列已被用于在体内监测大鼠骨骼肌中的肌酸激酶动力学。使用这些程序，正向通量（ATP合成）和反向通量（磷酸肌酸合成）经计算分别为8.98±0.6和10.7±0.8微摩尔/克湿重/秒（n = 5）。这些结果表明，在静息骨骼肌中，31P NMR光谱中观察到的大部分γ-ATP是胞质的，并与磷酸肌酸快速交换。高通量率反映了骨骼肌中肌酸激酶的高催化能力。
