Gower J D, Sayer J W, Wills E D
Biochem Pharmacol. 1986 Jun 15;35(12):1965-9. doi: 10.1016/0006-2952(86)90728-8.
The effect of varying the fatty acid composition of the lipid components of the diet on the activity of epoxide hydratase in the rat liver and intestinal mucosa has been studied. Feeding a 10% cod liver oil diet (containing 18% C20:5 and 11% C22:6) resulted in a 3-fold increase in epoxide hydratase activity in the liver and a 1.6-fold increase in the intestine compared to rats fed a fat-free diet. The activity of epoxide hydratase in rats fed a cod liver oil diet was significantly greater than that for the group fed a lard diet (containing mainly saturated and mono-unsaturated fatty acids) containing the same quantity of vitamin E. Thus, the enhancing effect of the cod liver oil diet was due to the polyunsaturated fatty acids in this oil. Dietary corn oil (58% C18:2) also stimulated epoxide hydratase activity in the liver but not in the intestine. Vitamin E levels of up to 500 mg/kg diet were ineffective at inducing epoxide hydratase activity in both the liver and intestine. Significant changes in the fatty acid composition of hepatic and intestinal microsomes took place when rats were fed diets of different fatty acid composition. These changes were such that the proportions of polyunsaturated fatty acids in the microsomal fractions reflected the amounts of these fatty acids in the dietary fat. Hepatic epoxide hydratase activity was found to be positively correlated to the proportion of polyunsaturated fatty acids in the microsomal fractions of the liver.
研究了改变饮食中脂质成分的脂肪酸组成对大鼠肝脏和肠黏膜中环氧水解酶活性的影响。与喂食无脂饮食的大鼠相比，喂食10%鱼肝油饮食（含18%二十碳五烯酸和11%二十二碳六烯酸）导致肝脏中环氧水解酶活性增加3倍，肠道中增加1.6倍。喂食鱼肝油饮食的大鼠中环氧水解酶的活性显著高于喂食含相同量维生素E的猪油饮食（主要含饱和脂肪酸和单不饱和脂肪酸）的组。因此，鱼肝油饮食的增强作用归因于该油中的多不饱和脂肪酸。膳食玉米油（58%十八碳二烯酸）也刺激了肝脏中环氧水解酶的活性，但对肠道无刺激作用。饮食中维生素E含量高达500mg/kg时，对肝脏和肠道中环氧水解酶活性的诱导均无效。当给大鼠喂食不同脂肪酸组成的饮食时，肝脏和肠道微粒体的脂肪酸组成发生了显著变化。这些变化使得微粒体部分中多不饱和脂肪酸的比例反映了膳食脂肪中这些脂肪酸的含量。发现肝脏环氧水解酶活性与肝脏微粒体部分中多不饱和脂肪酸的比例呈正相关。
