基于碳点和铽的核壳结构比率型荧光探针用于炭疽生物标志物的检测。
A core-shell structure ratiometric fluorescent probe based on carbon dots and Tb for the detection of anthrax biomarker.
机构信息
College of New Materials and Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China; Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology, Beijing 102617, China.
College of New Materials and Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Oct 15;299:122793. doi: 10.1016/j.saa.2023.122793. Epub 2023 May 3.
A novel core-shell structure ratiometric fluorescent probe was developed, which can selectively and sensitively detect 2,6-dipicolinic acid (DPA) as an anthrax biomarker. Carbon dots (CDs) was embedded into SiO nanoparticles, which was acted as an internal reference signal. Tb with green emission was connected to the carboxyl functionalized SiO, which was acted as a responsive signal. With the addition of DPA, the emission of CDs at 340 nm was unchanged, while the fluorescence of Tb at 544 nm was enhanced by the antenna effect. In the concentration range of 0.1-2 μM, the fluorescence intensity ratio of I/I showed a good linear relationship with the concentration of DPA, and the limit of detection (LOD) was 10.2 nM. In addition, the dual-emission probe showed an obvious fluorescence color change from colourless to green with increasing DPA under UV light, which enabled visual detection.
一种新型核壳结构比率荧光探针被开发出来，可选择性和灵敏性地检测 2,6-二吡啶酸（DPA）作为炭疽生物标志物。碳点（CDs）嵌入到 SiO2 纳米粒子中，作为内参信号。具有绿色发射的 Tb 连接到羧基功能化的 SiO2，作为响应信号。随着 DPA 的加入，CDs 在 340nm 处的发射强度保持不变，而 Tb 在 544nm 处的荧光由于天线效应而增强。在 0.1-2μM 的浓度范围内，I/I 的荧光强度比值与 DPA 的浓度呈良好的线性关系，检测限（LOD）为 10.2nM。此外，双发射探针在紫外光下随着 DPA 的增加，从无色到绿色显示出明显的荧光颜色变化，实现了可视化检测。
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