School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
Fujian Provincial Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.
Biosens Bioelectron. 2017 Aug 15;94:388-393. doi: 10.1016/j.bios.2017.03.027. Epub 2017 Mar 16.
In this work, a novel time-resolved ratiometric fluorescent probe based on dual lanthanide (Tb: terbium, and Eu: europium)-doped complexes (Tb/DPA@SiO-Eu/GMP) has been designed for detecting anthrax biomarker (dipicolinic acid, DPA), a unique and major component of anthrax spores. In such complexes-based probe, Tb/DPA@SiO can serve as a stable reference signal with green fluorescence and Eu/GMP act as a sensitive response signal with red fluorescence for ratiometric fluorescent sensing DPA. Additionally, the probe exhibits long fluorescence lifetime, which can significantly reduce the autofluorescence interferences from biological samples by using time-resolved fluorescence measurement. More significantly, a paper-based visual sensor for DPA has been devised by using filter paper embedded with Tb/DPA@SiO-Eu/GMP, and we have proved its utility for fluorescent detection of DPA, in which only a handheld UV lamp is used. In the presence of DPA, the paper-based visual sensor, illuminated by a handheld UV lamp, would result in an obvious fluorescence color change from green to red, which can be easily observed with naked eyes. The paper-based visual sensor is stable, portable, disposable, cost-effective and easy-to-use. The feasibility of using a smartphone with easy-to-access color-scanning APP as the detection platform for quantitative scanometric assays has been also demonstrated by coupled with our proposed paper-based visual sensor. This work unveils an effective method for accurate, sensitive and selective monitoring anthrax biomarker with backgroud-free and self-calibrating properties.
在这项工作中，设计了一种基于双镧系元素（Tb：铽和 Eu：铕）掺杂配合物（Tb/DPA@SiO-Eu/GMP）的新型时间分辨比率荧光探针，用于检测炭疽生物标志物（二吡咯羧酸，DPA），这是炭疽孢子的独特且主要成分。在这种基于配合物的探针中，Tb/DPA@SiO 可以作为具有绿色荧光的稳定参考信号，而 Eu/GMP 作为具有红色荧光的灵敏响应信号，用于 DPA 的比率荧光传感。此外，该探针具有长荧光寿命，通过使用时间分辨荧光测量，可以显著减少生物样品中的自发荧光干扰。更重要的是，通过使用嵌入 Tb/DPA@SiO-Eu/GMP 的滤纸设计了用于 DPA 的纸质可视化传感器，并且已经证明了其用于 DPA 的荧光检测的实用性，其中仅使用手持紫外灯。在存在 DPA 的情况下，手持紫外灯照射纸质可视化传感器会导致荧光颜色从绿色明显变为红色，肉眼即可轻松观察到。纸质可视化传感器稳定、便携、一次性、具有成本效益且易于使用。还通过与我们提出的纸质可视化传感器结合，展示了使用带有易于访问的彩色扫描 APP 的智能手机作为定量扫描测定的检测平台的可行性。这项工作揭示了一种用于准确、灵敏和选择性监测无背景和自校准特性的炭疽生物标志物的有效方法。
