Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Postdoctoral Research Station of Basic Medicine, The Affiliated Nanhua Hospital, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China.
Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Postdoctoral Research Station of Basic Medicine, The Affiliated Nanhua Hospital, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Oct 15;299:122802. doi: 10.1016/j.saa.2023.122802. Epub 2023 May 6.
Fabrication of a multi-signal readout assay with high sensitivity and selectivity is highly desirable for clinical and biochemical analysis, but remains a challenge due to laborious procedures, large-scale instruments, and inadequate accuracy. Herein, a straightforward, rapid, and portable detection platform based on palladium(II) methylene blue (MB) coordination polymer nanosheets (PdMBCP NSs) was unveiled for the ratiometric dual-mode detection of alkaline phosphatase (ALP) with temperature and colorimetric signal readout properties. The sensing mechanism is the ALP-catalyzed generation of ascorbic acid for competitive binding and etching PdMBCP NSs to release free MB in a quantitive means for detection. Specifically, ALP addition led to the decrease of temperature signal readout from the decomposed PdMBCP NSs under 808 nm laser excitation, and simultaneous increase of the temperature from the generated MB with a 660 nm laser, together with the corresponding absorbance changes at both wavelengths. Notably, this ratiometric nanosensor exhibited a detection limit of 0.013 U/L (colorimetric) and 0.095 U/L (photothermal) within 10 min, respectively. The reliability and satisfactory sensing performance of the developed method were further confirmed by clinic serum samples. Therefore, this study provides a new insight for the development of dual-signal sensing platforms for convenient, universal, and accurate detection of ALP.
制备具有高灵敏度和选择性的多信号读出分析物对于临床和生化分析非常重要，但由于繁琐的程序、大型仪器和不充分的准确性，这仍然是一个挑战。在此，我们揭示了一种基于钯 (II) 亚甲基蓝 (MB) 配位聚合物纳米片 (PdMBCP NSs) 的简单、快速且便携的检测平台，用于比率双模式检测碱性磷酸酶 (ALP)，具有温度和比色信号读出特性。传感机制是 ALP 催化生成抗坏血酸，用于竞争性结合和蚀刻 PdMBCP NSs，以定量方式释放游离 MB 进行检测。具体而言，ALP 的加入导致在 808nm 激光激发下分解的 PdMBCP NSs 的温度信号读出减少，同时由于生成的 MB 而产生的温度从 660nm 激光增加，以及在两个波长处的相应吸光度变化。值得注意的是，该比率纳米传感器在 10 分钟内分别显示出 0.013 U/L（比色）和 0.095 U/L（光热）的检测限。通过临床血清样本进一步证实了所开发方法的可靠性和令人满意的传感性能。因此，本研究为开发用于方便、通用和准确检测 ALP 的双信号传感平台提供了新的思路。
