Ge Xueli, Su Zhenguo, Wang Yuhao, Zhao Xue, Hou Kaifei, Zheng Shuna, Zeng Pengjiao, Shi Zhongqi, Hu Senhao, Wang Yuqing, Zhou Mengchen, Zhang Jiayu, Li Xiulian
School of Pharmacy, Binzhou Medical University, Yantai, China.
Affiliated Hospital of Binzhou Medical College, Yantai, China.
Front Nutr. 2023 Apr 28;10:1117460. doi: 10.3389/fnut.2023.1117460. eCollection 2023.
Polydatin is a biologically active compound found in mulberries, grapes, and , and it has uric acid-lowering effects. However, its urate-lowering effects and the molecular mechanisms underlying its function require further study.
In this study, a hyperuricemic rat model was established to assess the effects of polydatin on uric acid levels. The body weight, serum biochemical indicators, and histopathological parameters of the rats were evaluated. A UHPLC-Q-Exactive Orbitrap mass spectrometry-based metabolomics approach was applied to explore the potential mechanisms of action after polydatin treatment.
The results showed a trend of recovery in biochemical indicators after polydatin administration. In addition, polydatin could alleviate damage to the liver and kidneys. Untargeted metabolomics analysis revealed clear differences between hyperuricemic rats and the control group. Fourteen potential biomarkers were identified in the model group using principal component analysis and orthogonal partial least squares discriminant analysis. These differential metabolites are involved in amino acid, lipid, and energy metabolism. Of all the metabolites, the levels of L-phenylalanine, L-leucine, -butanoylcarnitine, and dihydroxyacetone phosphate decreased, and the levels of L-tyrosine, sphinganine, and phytosphingosine significantly increased in hyperuricemic rats. After the administration of polydatin, the 14 differential metabolites could be inverted to varying degrees by regulating the perturbed metabolic pathway.
This study has the potential to enhance our understanding of the mechanisms of hyperuricemia and demonstrate that polydatin is a promising potential adjuvant for lowering uric acid levels and alleviating hyperuricemia-related diseases.
白藜芦醇苷是一种存在于桑葚、葡萄等中的生物活性化合物，具有降尿酸作用。然而，其降尿酸作用及其功能的分子机制仍需进一步研究。
本研究建立高尿酸血症大鼠模型，以评估白藜芦醇苷对尿酸水平的影响。评估大鼠的体重、血清生化指标和组织病理学参数。采用基于超高效液相色谱-四极杆-静电场轨道阱质谱的代谢组学方法，探讨白藜芦醇苷治疗后的潜在作用机制。
结果显示，给予白藜芦醇苷后生化指标有恢复趋势。此外，白藜芦醇苷可减轻肝肾损伤。非靶向代谢组学分析显示高尿酸血症大鼠与对照组之间存在明显差异。通过主成分分析和正交偏最小二乘法判别分析，在模型组中鉴定出14种潜在生物标志物。这些差异代谢物参与氨基酸、脂质和能量代谢。在所有代谢物中，高尿酸血症大鼠的L-苯丙氨酸、L-亮氨酸、丁酰肉碱和磷酸二羟丙酮水平降低，L-酪氨酸、鞘氨醇和植物鞘氨醇水平显著升高。给予白藜芦醇苷后，通过调节紊乱的代谢途径，14种差异代谢物可在不同程度上发生逆转。
本研究有可能增进我们对高尿酸血症机制的理解，并证明白藜芦醇苷是一种有前途的潜在佐剂，可降低尿酸水平并减轻与高尿酸血症相关的疾病。
