Vetrivel Cittrarasu, Sivarasan Ganesan, Durairaj Kaliannan, Ragavendran Chinnasamy, Kamaraj Chinnaperumal, Karthika Sankar, Lo Huang-Mu
Carbon Capture Lab, Department of Chemical Engineering, SSN College of Engineering Kalavakkam, Chennai 603110, Tamil Nadu, India.
Department of Anatomy, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai 600077, Tamil Nadu, India.
Diagnostics (Basel). 2023 Apr 18;13(8):1464. doi: 10.3390/diagnostics13081464.
In order to support biomolecule attachment, an effective electrochemical transducer matrix for biosensing devices needs to have many specialized properties, including quick electron transfer, stability, high surface area, biocompatibility, and the presence of particular functional groups. Enzyme-linked immunosorbent assays, gel electrophoresis, mass spectrometry, fluorescence spectroscopy, and surface-enhanced Raman spectroscopy are common techniques used to assess biomarkers. Even though these techniques provide precise and trustworthy results, they cannot replace clinical applications because of factors such as detection time, sample amount, sensitivity, equipment expense, and the need for highly skilled individuals. For the very sensitive and targeted electrochemical detection of the salivary oral cancer biomarker IL8, we have created a flower-structured molybdenum disulfide-decorated zinc oxide composite on GCE (interleu-kin-8). This immunosensor shows very fast detection; the limit of detection (LOD) for interleukin-8 (IL8) detection in a 0.1 M phosphate buffer solution (PBS) was discovered to be 11.6 fM, while the MoS/ZnO nanocomposite modified glassy carbon electrode (GCE) demonstrated a high catalytic current linearly from 500 pg to 4500 pg mL interleukin-8 (IL8). Therefore, the proposed biosensor exhibits excellent stability, high accuracy sensitivity, repeatability, and reproducibility and shows the acceptable fabrication of the electrochemical biosensors to detect the ACh in real sample analysis.
为了支持生物分子附着，用于生物传感装置的有效电化学换能器基质需要具备许多特殊性质，包括快速电子转移、稳定性、高表面积、生物相容性以及特定官能团的存在。酶联免疫吸附测定、凝胶电泳、质谱、荧光光谱和表面增强拉曼光谱是用于评估生物标志物的常用技术。尽管这些技术能提供精确且可靠的结果，但由于检测时间、样品量、灵敏度、设备费用以及对高技能人员的需求等因素，它们无法替代临床应用。为了对唾液口腔癌生物标志物白细胞介素8（IL8）进行非常灵敏且有针对性的电化学检测，我们在玻碳电极（GCE）上制备了一种花状结构的二硫化钼修饰氧化锌复合材料。这种免疫传感器检测速度非常快；在0.1 M磷酸盐缓冲溶液（PBS）中检测白细胞介素8（IL8）的检测限（LOD）被发现为11.6 fM，而二硫化钼/氧化锌纳米复合材料修饰的玻碳电极（GCE）在白细胞介素8（IL8）浓度从500 pg至4500 pg mL范围内呈现出高催化电流线性关系。因此，所提出的生物传感器具有出色的稳定性、高精度灵敏度、重复性和再现性，并展示了在实际样品分析中用于检测乙酰胆碱的电化学生物传感器的可接受制备方法。
