Venugopal Divyambika Catakapatri, Caleb Cynthia Leslie, Kirupakaran Nandhini Priyadarshini, Shyamsundar Vidyarani, Ravindran Soundharya, Yasasve Madhavan, Krishnamurthy Arvind, Harikrishnan Thamizhchelvan, Sankarapandian Sathasivasubramanian, Ramshankar Vijayalakshmi
Department of Oral Medicine and Radiology, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai 600116, India.
Centre for Oral Cancer Prevention and Research, Sree Balaji Dental College and Hospital, Pallikaranai, Chennai 600100, India.
Biomedicines. 2023 Mar 28;11(4):1040. doi: 10.3390/biomedicines11041040.
Oral submucous fibrosis (OSMF) is highly prevalent in South East Asia with higher rates of malignant transformation in Indian subcontinent. Numerous biomarkers are now being studied to predict disease prognosis and detect malignant alterations at an early stage. Patients with clinically and biopsy-proven oral submucous fibrosis and oral squamous cell carcinoma were included in the study as the experimental group, while patients without a tobacco or betel nut habit who had their third molars surgically removed were included as the healthy control group. For the immunohistochemistry (IHC) investigation, 5-μm slices from formalin-fixed, paraffin-embedded tissue blocks (FFPE) were obtained. Fresh tissues ( = 45) from all three groups were collected and gene expression was studied using relative quantitation-based qPCR. The protein expression of octamer-binding transcription factor 3/4 (OCT 3/4) and sex-determining region Y-box 2 (SOX 2) was evaluated in the experimental group and compared with healthy controls. The IHC results showed a significant correlation with the expression of OCT 3/4 ( value = 0.000; χ2 = 20.244) and SOX 2 ( value = 0.006; χ2 = 10.101) among OSCC and OSMF patients in comparison to healthy controls. Both OCT 3/4 and SOX 2 showed overexpression of four-fold and three-fold in OSMF when compared to OSCC and healthy controls, respectively. This study shows the significant importance of cancer stem cell markers OCT 3/4 and SOX 2 to assess the disease prognosis in OSMF.
口腔黏膜下纤维化（OSMF）在东南亚地区高度流行，在印度次大陆的恶变率更高。目前正在研究众多生物标志物，以预测疾病预后并在早期检测恶性改变。经临床和活检证实的口腔黏膜下纤维化及口腔鳞状细胞癌患者被纳入研究作为实验组，而无烟草或槟榔习惯且接受过第三磨牙手术切除的患者被纳入作为健康对照组。对于免疫组织化学（IHC）研究，从福尔马林固定、石蜡包埋组织块（FFPE）中获取5μm切片。收集所有三组的新鲜组织（n = 45），并使用基于相对定量的qPCR研究基因表达。在实验组中评估八聚体结合转录因子3/4（OCT 3/4）和性别决定区Y盒2（SOX 2）的蛋白表达，并与健康对照组进行比较。免疫组织化学结果显示，与健康对照组相比，OSCC和OSMF患者中OCT 3/4（p值 = 0.000；χ2 = 20.244）和SOX 2（p值 = 0.006；χ2 = 10.101）的表达存在显著相关性。与OSCC和健康对照组相比，OSMF中OCT 3/4和SOX 2分别显示出四倍和三倍的过表达。本研究表明癌症干细胞标志物OCT 3/4和SOX 2在评估OSMF疾病预后方面具有重要意义。
