Lorge P, Cabiaux V, Long L, Ruysschaert J M
Biochim Biophys Acta. 1986 Jun 26;858(2):312-6. doi: 10.1016/0005-2736(86)90337-8.
We demonstrate here that fusion occurs between the membrane of the Newcastle disease virus (NDV) and liposomes. Fluorescence dequenching studies (using Rhodamine-bearing viral envelopes) revealed the mixing of the lipids constituting the viral and liposomal membrane. The digestion of internal viral proteins by trypsin-containing liposomes indicated the mixing of the internal aqueous compartments. This last assay is independent of exchange of lipids between liposomal and viral membrane in the absence of fusion. Investigation of the effects of liposomal composition indicated that the presence of phosphatidylethanolamine and gangliosides are essential to optimize fusion. The fact that the Newcastle disease virus membrane can fuse with liposome also confirms that fusion must be determined by the viral proteins and could be mostly independent of the nature or presence of the host proteins.
我们在此证明新城疫病毒（NDV）的膜与脂质体之间会发生融合。荧光猝灭研究（使用带有罗丹明的病毒包膜）揭示了构成病毒膜和脂质体膜的脂质发生了混合。含胰蛋白酶的脂质体对病毒内部蛋白质的消化表明内部水相区室发生了混合。在不存在融合的情况下，最后这项检测与脂质体膜和病毒膜之间脂质的交换无关。对脂质体组成影响的研究表明，磷脂酰乙醇胺和神经节苷脂的存在对于优化融合至关重要。新城疫病毒膜能够与脂质体融合这一事实也证实，融合必定由病毒蛋白决定，并且可能在很大程度上与宿主蛋白的性质或存在与否无关。
