Yamamoto T, Iwasaki Y, Konno H, Iizuka H
Brain Res. 1986 May 28;374(2):419-24. doi: 10.1016/0006-8993(86)90442-7.
There are certain limitations in identifying cells undergoing physiological neuronal death (PND) by conventional histological methods. Here, the Fink-Heimer method for impregnation of degenerating axons and terminals was successfully employed in selectively visualizing PND in the neonatal rat brain. The cell bodies thus observed were in close correlation with the known distribution and time-course of PND. In addition, massive occurrences of PND were observed in the periaqueductal gray, inferior colliculus, hypothalamus, and the rostral portion of the hippocampus, findings hitherto undescribed. Thus, the Fink-Heimer method provides a useful adjunct to the study of PND in the neonatal period.
通过传统组织学方法识别经历生理性神经元死亡(PND)的细胞存在一定局限性。在此,用于浸染退化轴突和终末的芬克-海默法成功应用于选择性观察新生大鼠脑中的PND。如此观察到的细胞体与已知的PND分布和时间进程密切相关。此外,在导水管周围灰质、下丘、下丘脑和海马体的吻侧部分观察到大量PND发生,这些发现迄今尚未见描述。因此,芬克-海默法为新生儿期PND的研究提供了一种有用的辅助手段。