Compounds from myrtle flowers as antibacterial agents and SARS-CoV-2 inhibitors: and molecular docking studies.

Plants and their related phytochemicals play a key role in the treatment of bacterial and viral infections, which inspire scientists to design and develop more efficient drugs starting from the phytochemical active scaffold. This work aims to characterize the chemical compounds of essential oil (EO) from Algeria and to evaluate its antibacterial effect, as well as the anti-SARS-CoV-2 activity. The chemical profile of hydrodistilled EO from myrtle flowers was determined using GC/MS analysis. The results showed qualitative and quantitative fluctuations and 54 compounds were identified including the main components: α-pinene (48.94%) and 1,8-cineole (28.3%) whereas other minor compounds were detected. The antibacterial activity of myrtle EO against Gram-negative bacteria was carried out by using the disc diffusion method. The best inhibition zone values ranged between 11 and 25 mm. The results revealed that (25 mm), (20 mm) and (20 mm) are the most susceptible strains to the EO which is endowed with a bactericidal effect. Furthermore, the antibacterial and anti-SARS-CoV-2 activities were investigated by the means of molecular docking (MD) study, in addition to ADME(Tox) analysis. The phytochemicals were docked against four targets: topoisomerase II DNA gyrase B (PDB: 1KZN), SARS-CoV-2 Main protease (PDB: 6LU7), Spike (PDB: 6ZLG) and angiotensin-converting enzyme II ACE2 (PDB: 1R42). The MD investigation revealed that 1,8-cineole could be the main phytochemical associated with the antibacterial activity of EO; s-cbz-cysteine, mayurone and methylxanthine were found the most promising phytochemicals against SARS-CoV-2; The ADME(Tox) analysis has shown their good druggability with no Lipinski's rule violation.