环状 PSMC3 通过调控 miR-31-5p/YAP/β-连环蛋白抑制结直肠癌细胞生长和迁移。
CircPSMC3 Inhibits Colorectal Cancer Cell Growth and Migration by Controlling miR-31-5p/YAP/β-Catenin.
机构信息
Department of General Surgery, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, 310012, China.
Department of Anesthesiology, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310052, China.
出版信息
Protein Pept Lett. 2023;30(6):469-476. doi: 10.2174/0929866530666230516143823.
INTRODUCTION
Colorectal cancer (CRC) is hackneyed cancer and a major lethiferous cancer. Circular RNAs (CircRNAs) have been discovered to own important roles in controlling CRC progression. CircPSMC3 is known to exhibit lower expression in diversified cancers. However, the regulatory function of CircPSMC3 in CRC keeps unclear.
METHODS
The expression of CircPSMC3 and miR-31-5p was confirmed through RT-qPCR. The cell proliferation was measured through CCK-8 and EdU assays. The protein expression of genes was examined through a western blot. The cell invasion and migration were tested through Transwell and wound healing assays. The binding ability between CircPSMC3 and miR-31-5p was confirmed through the luciferase reporter assay.
RESULTS
CircPSMC3 exhibited lower expression in CRC tissues and cell lines. Additionally, CircPSMC3 was revealed to suppress cell proliferation in CRC. Moreover, through Transwell and wound healing assays, CircPSMC3 was discovered to repress CRC cell invasion and migration. In CRC tissues, miR-31-5p expression was up-regulated and negatively correlated with CircPSMC3 expression. Further mechanism exploration experiments disclosed that CircPSMC3 is bound with miR-31-5p to modulate the YAP/β-catenin axis in CRC. At last, through rescue assays, CircPSMC3 inhibited cell proliferation, invasion and migration through sponging miR-31-5p in CRC.
CONCLUSION
Our work was the first time to probe the potential regulatory effects of CircPSMC3 in CRC, and these above results uncovered that CircPSMC3 inhibited CRC cell growth and migration through regulating miR-31-5p/YAP/β-catenin. This discovery hinted that CircPSMC3 may serve as a useful therapeutic candidate for CRC.
简介
结直肠癌（CRC）是一种常见的癌症，也是一种主要的致命性癌症。环状 RNA（CircRNAs）已被发现在控制 CRC 进展中具有重要作用。CircPSMC3 在多种癌症中表达水平较低。然而，CircPSMC3 在 CRC 中的调控功能尚不清楚。
方法
通过 RT-qPCR 验证 CircPSMC3 和 miR-31-5p 的表达。通过 CCK-8 和 EdU 测定法测量细胞增殖。通过 Western blot 检测基因的蛋白表达。通过 Transwell 和划痕愈合实验测试细胞侵袭和迁移。通过荧光素酶报告实验验证 CircPSMC3 与 miR-31-5p 之间的结合能力。
结果
CircPSMC3 在 CRC 组织和细胞系中表达水平较低。此外，CircPSMC3 被证明可抑制 CRC 细胞增殖。此外，通过 Transwell 和划痕愈合实验发现，CircPSMC3 可抑制 CRC 细胞侵袭和迁移。在 CRC 组织中，miR-31-5p 的表达上调，并与 CircPSMC3 表达呈负相关。进一步的机制探索实验表明，CircPSMC3 与 miR-31-5p 结合，调节 CRC 中的 YAP/β-catenin 轴。最后，通过挽救实验，CircPSMC3 通过海绵吸附 miR-31-5p 在 CRC 中抑制细胞增殖、侵袭和迁移。
结论
我们的工作首次探讨了 CircPSMC3 在 CRC 中的潜在调节作用，这些结果表明 CircPSMC3 通过调节 miR-31-5p/YAP/β-catenin 抑制 CRC 细胞的生长和迁移。这一发现暗示 CircPSMC3 可能成为 CRC 的一种有用的治疗候选物。
Zotero 插件