基于四面体 DNA 信号放大的可重复使用电化学发光生物传感器用于超灵敏检测 microRNAs。
Reusable electrochemiluminescence biosensor based on tetrahedral DNA signal amplification for ultrasensitive detection of microRNAs.
机构信息
Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing 210023, China.
School of Geographic and Biologic Information, Nanjing University of Posts and Telecommunications, Nanjing 210023, China.
出版信息
Chem Commun (Camb). 2023 Jun 1;59(45):6869-6872. doi: 10.1039/d3cc01830j.
A novel and reusable electrochemiluminescence biosensor was developed based on tetrahedral DNA (TDN) signal amplification for ultrasensitive detection of miRNA-27a. The flowered nickel-iron layered double hydroxide@AuNPs (NiFe-LDH@AuNPs) composites increase the amount of hairpin DNA fixed on the electrode. When miRNA is present, TDN-Ru(bpy) acts as an ECL probe, forming a stable sandwich structure with miRNA-27a and hairpin DNA through base complementation pairing, thus achieving miRNA detection. This biosensor has the characteristics of high sensitivity, excellent selectivity, and good reproducibility.
基于四面体 DNA（TDN）信号放大，开发了一种新颖且可重复使用的电化学发光生物传感器，用于超灵敏检测 miRNA-27a。花状镍铁层状双氢氧化物@AuNPs（NiFe-LDH@AuNPs）复合材料增加了固定在电极上的发夹 DNA 的数量。当存在 miRNA 时，TDN-Ru(bpy) 作为 ECL 探针，通过碱基互补配对与 miRNA-27a 和发夹 DNA 形成稳定的三明治结构，从而实现 miRNA 的检测。该生物传感器具有高灵敏度、优异的选择性和良好的重现性。
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