Santo Claudio Ignazio, Conejo-Cuevas Guillermo, Paolucci Francesco, Del Campo Francisco Javier, Valenti Giovanni
Department of Chemistry "G.Ciamician", University of Bologna, UE4, Via. P. Gobetti 85, 40129 Bologna, Italy.
BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, Vizcaya Spain.
Chem Biomed Imaging. 2024 Nov 22;2(12):835-841. doi: 10.1021/cbmi.4c00070. eCollection 2024 Dec 23.
Electrochemiluminescence (ECL) is nowadays a powerful technique widely used in biosensing and imaging, offering high sensitivity and specificity for detecting and mapping biomolecules. Screen-printed electrodes (SPEs) offer a versatile and cost-effective platform for ECL applications due to their ease of fabrication, disposability, and suitability for large-scale production. This research introduces a novel method for improving the ECL characteristics of screen-printed carbon electrodes (SPCEs) through the application of CO laser treatment following fabrication. Using advanced ECL microscopy, we analyze three distinct carbon paste-based electrodes and show that low-energy laser exposure (ranging from 7 to 12 mJ·cm) enhances the electrochemical performance of the electrodes. This enhancement results from the selective removal of surface binders and contaminants achieved by the laser treatment. We employed ECL microscopy to characterize the ECL emission using a bead-based system incorporating magnetic microbeads, like those used in commercial platforms. This approach enabled high-resolution spatial mapping of the electrode surface, offering valuable insights into its electrochemical performance. Through quantitative assessment using a photomultiplier tube (PMT), it was observed that GST electrodes could detect biomarkers with high sensitivity, achieving an approximate detection limit (LOD) of 11 antibodies per μm. These findings emphasize the potential of laser-modified GST electrodes in enabling highly sensitive electrochemiluminescent immunoassays and various biosensing applications.
如今，电化学发光（ECL）是一种广泛应用于生物传感和成像的强大技术，在检测和绘制生物分子方面具有高灵敏度和特异性。丝网印刷电极（SPEs）因其易于制造、可一次性使用且适合大规模生产，为ECL应用提供了一个多功能且具有成本效益的平台。本研究介绍了一种新颖的方法，即在制造后的丝网印刷碳电极（SPCEs）上应用CO激光处理来改善其ECL特性。使用先进的ECL显微镜，我们分析了三种不同的基于碳糊的电极，并表明低能量激光照射（范围为7至12 mJ·cm）可增强电极的电化学性能。这种增强源于激光处理实现的表面粘合剂和污染物的选择性去除。我们采用ECL显微镜，利用包含磁性微珠的基于珠子的系统（类似于商业平台中使用的那些）来表征ECL发射。这种方法能够对电极表面进行高分辨率空间映射，为其电化学性能提供有价值的见解。通过使用光电倍增管（PMT）进行定量评估，观察到GST电极能够高灵敏度地检测生物标志物，实现了每μm约11个抗体的检测限（LOD）。这些发现强调了激光修饰的GST电极在实现高灵敏度电化学发光免疫测定和各种生物传感应用方面的潜力。
