Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, Houston, TX, 77030, United States of America.
Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, Houston, TX, 77030, United States of America; Department of Translational Medical Sciences, School of Medicine, Texas A&M University, Houston, TX, 77030, United States of America.
Cell Calcium. 2023 Jul;113:102755. doi: 10.1016/j.ceca.2023.102755. Epub 2023 May 10.
Genetic code expansion technology has been widely applied to control protein activity and biological systems by taking advantage of an amber stop codon suppressor tRNA and orthogonal aminoacyl-tRNA synthetase pair. With this chemical biology approach, Maltan et al. incorporated photocrosslinking unnatural amino acids (UAAs) into the transmembrane domains of ORAI1 to enable UV light-inducible calcium influx across the plasma membrane, mechanistic interrogation of the calcium release-activated calcium (CRAC) channel at the single amino acid level, and remote control of downstream calcium-modulated signaling in mammalian cells.
遗传密码扩展技术通过利用琥珀终止密码子抑制 tRNA 和正交氨酰-tRNA 合成酶对来控制蛋白质活性和生物系统。利用这种化学生物学方法，Maltan 等人将光交联非天然氨基酸 (UAA) 掺入 ORAI1 的跨膜结构域，使钙离子能够在质膜上进行光诱导的内流，在单个氨基酸水平上对钙释放激活钙 (CRAC) 通道进行机械探究，以及对哺乳动物细胞中钙调节信号的下游进行远程控制。
