Cellular Biology Laboratory, Institute of Nutrition and Food Technology (INTA), University of Chile, Chile; OMEGA Laboratory, Institute of Nutrition and Food Technology (INTA), University of Chile, Chile.
Cellular Biology Laboratory, Institute of Nutrition and Food Technology (INTA), University of Chile, Chile.
Biochim Biophys Acta Mol Basis Dis. 2023 Aug;1869(6):166749. doi: 10.1016/j.bbadis.2023.166749. Epub 2023 May 15.
Metabolic alterations associated with obesity have been related to chronodisruption i.e., the desynchronization of molecular clocks that regulate circadian rhythms. The search for tools that improve the dietary treatment of obesity has recently focused on behaviors related to chronodisruption, and intermittent fasting is increasingly gaining interest. Studies in animal models have identified the benefits of time-restricted feeding (TRF) on metabolic alterations associated with changes in circadian rhythms induced by a high-fat diet. We aimed to evaluate the effect of TRF in flies with metabolic damage and chronodisruption.
Using high-fat diet fed Drosophila melanogaster as a model of metabolic damage and chronodisruption, we determined the impact of 12-h TRF on metabolic and molecular markers. Flies with metabolic dysfunction were switched to a control diet and randomly assigned to Ad libitum or a TRF regimen for seven days. We evaluated total triglyceride content, glycemia, weight, and 24 h mRNA expression rhythms of Nlaz (insulin resistance marker), clock genes (circadian rhythm molecular markers), and the neuropeptide Cch-amide2.
Flies with metabolic damage that received TRF showed lower total triglyceride content, Nlaz expression, circulating glucose, and weight compared to Ad libitum. We observed the recovery of some of the high-fat diet-induced alterations in the amplitude of the circadian rhythm, particularly in the peripheral clock.
TRF produced a partial reversal of metabolic dysfunction and chronodisruption of circadian cycles.
TRF could be a useful tool to help to ameliorate metabolic and chronobiologic damage induced by a high-fat diet.
与肥胖相关的代谢改变与生物钟紊乱有关，即调节昼夜节律的分子钟失同步。寻找改善肥胖饮食治疗的工具最近集中在与生物钟紊乱相关的行为上，间歇性禁食越来越受到关注。动物模型研究已经确定了限时喂养（TRF）对高脂肪饮食引起的昼夜节律变化相关代谢改变的益处。我们旨在评估 TRF 对代谢损伤和生物钟紊乱果蝇的影响。
我们使用高脂肪饮食喂养的黑腹果蝇作为代谢损伤和生物钟紊乱的模型，确定 12 小时 TRF 对代谢和分子标志物的影响。代谢功能障碍的果蝇切换到对照饮食，并随机分为自由进食或 TRF 方案 7 天。我们评估了总甘油三酯含量、血糖、体重和 24 小时 Nlaz（胰岛素抵抗标志物）、时钟基因（昼夜节律分子标志物）和神经肽 Cch-amide2 的 mRNA 表达节律。
接受 TRF 的代谢损伤果蝇的总甘油三酯含量、Nlaz 表达、循环葡萄糖和体重均低于自由进食组。我们观察到高脂肪饮食诱导的昼夜节律振幅的一些改变得到了恢复，特别是在外周时钟。
TRF 部分逆转了代谢功能障碍和昼夜节律周期的生物钟紊乱。
TRF 可能是一种有用的工具，可以帮助改善高脂肪饮食引起的代谢和 chronobiologic 损伤。
