Laboratório de Genética & Biodiversidade, Universidade Federal de Goiás, Goiânia, GO, Brazil.
Instituto Federal de Goiás - Campus Cidade de Goiás, Goiás, GO, Brazil.
Gene. 2023 Aug 5;876:147488. doi: 10.1016/j.gene.2023.147488. Epub 2023 May 16.
Myrteae is the most diversified tribe in the Myrtaceae family and has great ecological and economic importance. Here, we performed the assembly and annotation of the chloroplast genome of Eugenia klotzschiana O. Berg and used this in a comparative analysis with other 13 species from the Myrteae tribe. The E. klotzschiana plastome exhibited a length of 158,977 bp and a very conserved structure and gene composition when compared with other Myrteae genomes. We identified 34 large repetitive sequences and 94 SSR repeats in E. klotzschiana plastome. The trnT-trnL, rpl32-trnL, ndhF-rpl32, psbE-petL, and ycf1 regions were identified as mutational hotspots. A negative selection signal was detected in 74 protein-coding genes while neutral evolution was detected in two genes (rps12 and psaI). Furthermore, 222 RNA editing sites were identified in the E. klotzschiana plastome. We also obtained a plastome-based Myrtales phylogenetic tree, including E. klotzschiana for the first time in a molecular phylogeny, recovering its sister relationship for all other Eugenia species. Our results illuminate how evolution shaped the chloroplast genome structure and composition in the Myrteae tribe, especially in the E. klotzschiana plastome.
桃金娘科的桃金娘族是最具多样性的一个族，具有重要的生态和经济意义。在这里，我们对嘉赐树 Eugenia klotzschiana O. Berg 的叶绿体基因组进行了组装和注释，并与桃金娘族的其他 13 个物种进行了比较分析。与其他桃金娘族基因组相比，E. klotzschiana 质体基因组长度为 158977 bp，具有非常保守的结构和基因组成。我们在 E. klotzschiana 质体基因组中鉴定出 34 个大重复序列和 94 个 SSR 重复。trnT-trnL、rpl32-trnL、ndhF-rpl32、psbE-petL 和 ycf1 区被鉴定为突变热点。在 74 个蛋白质编码基因中检测到负选择信号，而在两个基因（rps12 和 psaI）中检测到中性进化。此外，在 E. klotzschiana 质体基因组中还鉴定出 222 个 RNA 编辑位点。我们还获得了基于质体的桃金娘目系统发育树，这是首次在分子系统发育中包括 E. klotzschiana，它与所有其他 Eugenia 物种的姐妹关系得到了恢复。我们的研究结果阐明了进化如何塑造桃金娘族叶绿体基因组的结构和组成，特别是在 E. klotzschiana 质体基因组中。
