Goodman J W, Shinpock S G, Basford N L
Exp Hematol. 1979 Jan;7(1):17-26.
Because of mounting evidence of involvement of thymus-derived cells in blood formation we have studied the growth of transplanted bone marrow in mice extensively depleted of T-lymphocytes (TCD). Poor growth of parental marrow was found not to be appreciably altered in TCD hybrid recipients. However, parental thymic lymphocytes even in massive doses were not able to augment hemopoiesis in TCD hosts, in contrast to findings from sham-thymectomized or age control mice. This indication that a third (host) cell takes part in the thymocyte-marrow stem cell interaction was reinforced by the finding that isogenic (hybrid) thymocytes administered to TCD mice 5 weeks before the final irradiation restored their ability to support thymocyte-induced augmentation of parental marrow growth. Data were obtained from theta-poor sham-thymectomized irradiated controls which are interpreted as evidence for a suppressor T cell. Thymocytes administered with marrow produced a shift toward granulopoiesis in TCD mice as well as in controls. From this finding we infer that although thymus-derived cells are intimately involved in regulation of myelopoiesis, the effect of administered thymic lymphocytes on the differentiative pathway does not depend on host T cells.
由于越来越多的证据表明胸腺来源的细胞参与血液形成，我们对严重缺乏T淋巴细胞（TCD）的小鼠体内移植骨髓的生长进行了广泛研究。我们发现，在TCD杂交受体中，亲代骨髓的生长不佳并没有明显改变。然而，与假胸腺切除或年龄对照小鼠的结果相反，即使给予大量亲代胸腺淋巴细胞也无法增强TCD宿主的造血功能。给TCD小鼠在最终照射前5周注射同基因（杂交）胸腺细胞，恢复了它们支持胸腺细胞诱导亲代骨髓生长增强的能力，这一发现进一步证明了第三种（宿主）细胞参与了胸腺细胞与骨髓干细胞的相互作用。从缺乏θ细胞的假胸腺切除照射对照中获得的数据被解释为存在抑制性T细胞的证据。与骨髓一起注射的胸腺细胞在TCD小鼠以及对照小鼠中都导致了向粒细胞生成的转变。从这一发现我们推断，尽管胸腺来源的细胞密切参与骨髓生成的调节，但注射的胸腺淋巴细胞对分化途径的影响并不依赖于宿主T细胞。
