UWA School of Agriculture and Environment, The University of Western Australia, Crawley, Western Australia, Australia.
The UWA Institute of Agriculture, The University of Western Australia, Crawley, Western Australia, Australia.
Plant Genome. 2024 Mar;17(1):e20343. doi: 10.1002/tpg2.20343. Epub 2023 May 18.
Drought is a major constraint for wheat production that is receiving increased attention due to global climate change. This study conducted isobaric tags for relative and absolute quantitation proteomic analysis on near-isogenic lines to shed light on the underlying mechanism of qDSI.4B.1 quantitative trait loci (QTL) on the short arm of chromosome 4B conferring drought tolerance in wheat. Comparing tolerant with susceptible isolines, 41 differentially expressed proteins were identified to be responsible for drought tolerance with a p-value of < 0.05 and fold change >1.3 or <0.7. These proteins were mainly enriched in hydrogen peroxide metabolic activity, reactive oxygen species metabolic activity, photosynthetic activity, intracellular protein transport, cellular macromolecule localization, and response to oxidative stress. Prediction of protein interactions and pathways analysis revealed the interaction between transcription, translation, protein export, photosynthesis, and carbohydrate metabolism as the most important pathways responsible for drought tolerance. The five proteins, including 30S ribosomal protein S15, SRP54 domain-containing protein, auxin-repressed protein, serine hydroxymethyltransferase, and an uncharacterized protein with encoding genes on 4BS, were suggested as candidate proteins responsible for drought tolerance in qDSI.4B.1 QTL. The gene coding SRP54 protein was also one of the differentially expressed genes in our previous transcriptomic study.
干旱是小麦生产的主要限制因素，由于全球气候变化，这一问题受到了越来越多的关注。本研究利用等压标签相对和绝对定量技术对近等基因系进行蛋白质组学分析，以揭示赋予小麦耐旱性的 4B 染色体短臂 qDSI.4B.1 数量性状位点（QTL）的潜在机制。在耐旱与易感近等基因系之间进行比较，鉴定出 41 个差异表达蛋白，这些蛋白与耐旱性有关，p 值<0.05，倍数变化>1.3 或<0.7。这些蛋白主要富集在过氧化氢代谢活性、活性氧代谢活性、光合作用、细胞内蛋白质运输、细胞大分子定位和对氧化应激的反应中。蛋白质相互作用和途径分析的预测表明，转录、翻译、蛋白质输出、光合作用和碳水化合物代谢之间的相互作用是与耐旱性最相关的重要途径。包括 30S 核糖体蛋白 S15、SRP54 结构域蛋白、生长素抑制蛋白、丝氨酸羟甲基转移酶和一个在 4BS 上具有编码基因的未知功能蛋白在内的 5 种蛋白被认为是 qDSI.4B.1 QTL 中耐旱性的候选蛋白。编码 SRP54 蛋白的基因也是我们之前转录组研究中差异表达的基因之一。
