National Engineering Technology Research Center for Combined Vaccines, Wuhan, China.
Gansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Lanzhou, China.
Expert Rev Vaccines. 2023 Jan-Dec;22(1):468-480. doi: 10.1080/14760584.2023.2214219.
Inactivated virus vaccines are the most widely used tool to prevent disease. To meet vaccine production demands, increasing attention has been placed on identifying methods to improve vaccine production efficiency. The use of suspended cells can greatly increase vaccine production. Suspension acclimation is a traditional method to convert adherent cells to suspension strains. Furthermore, as genetic engineering technology has developed, increasing attention has focused on the development of suspension cell lines using targeted genetic engineering techniques.
This review systematically summarizes and analyzes the development and research progress of various inactivated viral vaccine production suspension cell lines and provides protocols and candidate target genes for the engineered establishment of additional suspension cell lines for vaccine production.
The use of suspended cells can significantly improve the production efficiency of inactivated virus vaccines and other biological products. Presently, cell suspension culture is the key component to improve many vaccine production processes.
灭活病毒疫苗是预防疾病最广泛使用的工具。为了满足疫苗生产的需求，人们越来越关注寻找提高疫苗生产效率的方法。悬浮细胞的使用可以极大地增加疫苗的产量。悬浮适应是将贴壁细胞转化为悬浮株的传统方法。此外，随着基因工程技术的发展，越来越多的注意力集中在使用靶向基因工程技术开发悬浮细胞系上。
本文系统地总结和分析了各种灭活病毒疫苗生产悬浮细胞系的发展和研究进展，并为工程化建立更多用于疫苗生产的悬浮细胞系提供了方案和候选靶基因。
悬浮细胞的使用可以显著提高灭活病毒疫苗和其他生物制品的生产效率。目前，细胞悬浮培养是提高许多疫苗生产工艺的关键组成部分。
