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对 M. Bieb 的广泛评估:化学成分和元素组成、总酚含量和抗菌活性分析,以及自由基清除潜力的量子化学计算。

A broad assessment of M. Bieb: chemical and elemental composition, total phenolic and antimicrobial activity analysis, and quantum chemical calculations of radical scavenging potential.

机构信息

Faculty of Arts and Science, Department of Chemistry, Siirt University, Siirt, Turkey.

Faculty of Engineering, Department of Food Engineering, Siirt University, Siirt, Turkey.

出版信息

Int J Environ Health Res. 2024 Apr;34(4):2124-2139. doi: 10.1080/09603123.2023.2214100. Epub 2023 May 18.

DOI:10.1080/09603123.2023.2214100
PMID:37199334
Abstract

Eremurus spectabilis M. Bieb was extensively investigated experimentally and theoretically, including the antioxidant properties of compounds such as flavonoids, hydroxycinnamic acid derivatives, hydroxybenzoic acid derivatives, and organic acids. Antioxidant activity was investigated using the Density Functional Theory (DFT) method based on three known mechanisms: hydrogen atom transfer (HAT), single electron transfer followed by proton transfer (SET-PT), and sequential proton loss electron transfer (SPLET). Subcritical water extraction (SWE), soxhlet extraction (SE), and solvent extraction (SOE) techniques were applied in the extraction process. Malic acid was the major compound with an extract concentration of 38,532.84 ± 1849.58 µg analyte/kg, total phenolics, and free radical scavenging activity were 10.67 mg gallic acid/mL extract and 73.89% per mg/mL extract, respectively. P, Fe, Na, Mg, K, and Ca were the main elements. The antibacterial activity of E. spectabilis against seven bacteria was evaluated, and it was found to be higher than the commercial antibiotics P10 and AMC30.

摘要

美丽绿绒蒿(Eremurus spectabilis M. Bieb)被广泛地进行了实验和理论研究,包括类黄酮、羟基肉桂酸衍生物、羟基苯甲酸衍生物和有机酸等化合物的抗氧化特性。抗氧化活性采用基于三种已知机制的密度泛函理论(DFT)方法进行研究:氢原子转移(HAT)、单电子转移后质子转移(SET-PT)和顺序质子丢失电子转移(SPLET)。在提取过程中应用了亚临界水提取(SWE)、索氏提取(SE)和溶剂提取(SOE)技术。苹果酸是主要化合物,提取浓度为 38,532.84 ± 1849.58 µg 分析物/kg,总酚和自由基清除活性分别为 10.67 mg 没食子酸/mL 提取物和 73.89%/mg/mL 提取物。磷、铁、钠、镁、钾和钙是主要元素。评估了美丽绿绒蒿对七种细菌的抗菌活性,发现其抗菌活性高于商业抗生素 P10 和 AMC30。

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