RNA-binding protein promotes the tumor progression of gastric cancer by binding to and regulating gastric cancer-related genes.

BACKGROUND

This study sought to identify the downstream target genes of enolase 1 (), clarify the role of in gastric cancer (GC), and provide novel insights into the regulatory mechanisms of in the occurrence and development of GC.

METHODS

We performed RNA-immunoprecipitation sequencing in MKN-45 cells to study the types and abundance of pre-messenger RNA (mRNA)/mRNA bound by , the binding sites and motifs, the relationship between binding and its regulation of transcription level, and alternative splicing level by combining with RNA-sequencing (RNA-seq) data to further clarify the role of in GC.

RESULTS

We found that stabilized the expression of SRY-box transcription factor 9 (), vascular endothelial growth factor A (), G protein-coupled receptor class C group 5 member A (), and myeloid cell leukemia-1 () by binding to their mRNA, which increased the growth of GC. In addition, interacted with some other long non-coding RNAs (lncRNAs) or small-molecule kinases, such as , , , and pyruvate kinase M2 (), to regulate their expression to affect cell proliferation, migration, and apoptosis.

CONCLUSIONS

may play a role in GC by binding to and regulating GC-related genes. Our findings extend understandings of its mechanism as a clinical therapeutic target.