College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China; Ningbo Research Institute, Zhejiang University, Ningbo 315100, People's Republic of China.
Food Chem. 2023 Oct 15;423:136330. doi: 10.1016/j.foodchem.2023.136330. Epub 2023 May 12.
This study aimed to improve the thermodynamic performance of nanoliposomes (NLs) using fucoidan (F) as the second-layer coating biopolymer along with chitosan (CS), to control the delivery and bioavailability of catechin (C) and juglone (J). The stabilized liposomal carrier of F/CS-conjugated JC-NL (F-CS-JC-NL) was developed with optimum concentrations of CS (0.09 wt%) and F (0.10 wt%), with the highest encapsulation efficiency of juglone (95.47%) and catechin (90.88%). Physicochemical characterization revealed that F-CS-JC-NL disclosed improved stability under different pH and ionic strengths, with the maximum juglone/catechin retention under thermal, oxidative and storage conditions. In vitro digestion revealed that NL double-coating (F-CS-JC-NL) significantly reduced compound leakage in the gastrointestinal tract, providing a controlled release and better bioavailability of juglone/catechin compared to CS-JC-NL and JC-NL. Conclusively, this study provides a novel NL-based delivery carrier with enhanced physicochemical stability and controlled release that might have promising use in delivering functional ingredients.
本研究旨在通过使用褐藻糖胶 (F) 作为第二层包覆生物聚合物与壳聚糖 (CS) 一起，提高纳米脂质体 (NL) 的热力学性能，以控制儿茶素 (C) 和胡桃醌 (J) 的递送和生物利用度。通过使用最佳浓度的 CS (0.09 wt%) 和 F (0.10 wt%)，开发了稳定的 F/CS 缀合 JC-NL 的脂质体载体 (F-CS-JC-NL)，具有最高的胡桃醌 (95.47%) 和儿茶素 (90.88%) 的包封效率。物理化学特性研究表明，F-CS-JC-NL 在不同 pH 值和离子强度下显示出改善的稳定性，在热、氧化和储存条件下，胡桃醌/儿茶素的保留率最高。体外消化研究表明，NL 双层包覆 (F-CS-JC-NL) 可显著减少胃肠道中化合物的泄漏，与 CS-JC-NL 和 JC-NL 相比，可提供胡桃醌/儿茶素的控制释放和更好的生物利用度。总之，本研究提供了一种新型基于 NL 的递送载体，具有增强的物理化学稳定性和控制释放，可能在递送功能性成分方面具有广阔的应用前景。
