College of Forestry, Northeast Forestry University, Harbin, Heilongjiang Province, China.
Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
J Sci Food Agric. 2023 May;103(7):3306-3314. doi: 10.1002/jsfa.12484. Epub 2023 Feb 18.
Numerous positive effects have been attributed to lutein, a lipophilic nutrient, including resisting ultraviolet radiation and protecting retinal pigment epithelial (RPE) cells against blue light damage. It also has preventive effects against cardiovascular disease and cancer. However, its use could be limited by its poor stability and low bioaccessibility in the human digestive system. An encapsulation delivery system was therefore developed to resolve these limitations. In this study, chitosan-modified lutein nanoliposomes (CS-LNLs), chitosan-EGCG covalently modified lutein nanoliposomes (C-CS-EGCG-LNLs), and chitosan-EGCG noncovalently modified lutein nanoliposomes (non-C-CS-EGCG-LNLs) were designed. The average particle size, ζ-potential, and retention of lutein during storage were measured to indicate the physicochemical stability of the modified lutein nanoliposomes. The bioaccessibility of modified lutein nanoliposomes was also investigated to demonstrate the availability of lutein in the human digestive system.
First, Fourier-transform infrared spectroscopy (FTIR) verified that covalent bonds between chitosan and EGCG were formed. Subsequently, ζ-potential results revealed that C-CS-EGCG-LNLs had a relatively stable structure in comparison with lutein nanoliposomes (LNLs). The retention rate of lutein in CS-LNLs, C-CS-EGCG-LNLs, and non-C-CS-EGCG-LNLs was improved, especially in C-CS-EGCG-LNLs (at around 70% of lutein in initial system). An in vitro digestion experiment illustrated that CS-LNLs, C-CS-EGCG-LNLs, and non-C-CS-EGCG-LNLs presented relatively higher bioaccessibility, especially in C-CS-EGCG-LNLs (at around 33% of luein in initial system), which increased 2.5 and 1.65 times in comparison with free lutein and LNLs, respectively.
Overall, the results showed that C-CS-EGCG-LNLs presented greater physicochemical stability and bioaccessibility than LNLs, CS-LNLs, and non-C-CS-EGCG-LNLs. © 2023 Society of Chemical Industry.
叶黄素是一种亲脂性营养素，具有抵抗紫外线和保护视网膜色素上皮 (RPE) 细胞免受蓝光损伤等诸多积极作用。它还具有预防心血管疾病和癌症的作用。然而，由于其在人体消化系统中的稳定性差和生物利用度低，其应用可能受到限制。因此，开发了一种包封传递系统来解决这些限制。在这项研究中，设计了壳聚糖修饰的叶黄素纳米脂质体 (CS-LNLs)、壳聚糖-EGCG 共价修饰的叶黄素纳米脂质体 (C-CS-EGCG-LNLs) 和壳聚糖-EGCG 非共价修饰的叶黄素纳米脂质体 (non-C-CS-EGCG-LNLs)。测量了叶黄素纳米脂质体在储存过程中的平均粒径、ζ-电位和保留率，以表明修饰的叶黄素纳米脂质体的物理化学稳定性。还研究了修饰的叶黄素纳米脂质体的生物利用度，以证明叶黄素在人体消化系统中的可用性。
首先，傅里叶变换红外光谱 (FTIR) 验证了壳聚糖和 EGCG 之间形成了共价键。随后，ζ-电位结果表明，与叶黄素纳米脂质体 (LNLs) 相比，C-CS-EGCG-LNLs 具有相对稳定的结构。壳聚糖修饰的叶黄素纳米脂质体 (CS-LNLs)、C-CS-EGCG-LNLs 和 non-C-CS-EGCG-LNLs 中的叶黄素保留率得到提高，特别是在 C-CS-EGCG-LNLs 中（约为初始系统中叶黄素的 70%）。体外消化实验表明，CS-LNLs、C-CS-EGCG-LNLs 和 non-C-CS-EGCG-LNLs 具有相对较高的生物利用度，特别是在 C-CS-EGCG-LNLs 中（约为初始系统中叶黄素的 33%），与游离叶黄素和 LNLs 相比，分别提高了 2.5 和 1.65 倍。
总的来说，结果表明，与 LNLs、CS-LNLs 和 non-C-CS-EGCG-LNLs 相比，C-CS-EGCG-LNLs 具有更好的物理化学稳定性和生物利用度。
