Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, Heilongjiang Province, College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, 150025, PR China.
Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, Heilongjiang Province, College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, 150025, PR China.
Environ Res. 2023 Aug 15;231(Pt 2):116164. doi: 10.1016/j.envres.2023.116164. Epub 2023 May 16.
The degradation of phenol from wastewater is crucial for environmental protection. Biological enzymes, such as horseradish peroxidase (HRP), have shown great potential in the degradation of phenol. In this research, we prepared a hollow CuO/CuO octahedron adsorbent with a carambola matrix shape through the hydrothermal method. The surface of the adsorbent was modified by silane emulsion self-assembly, where 3-aminophenyl boric acid (APBA) and polyoxometalate (PW) were combined with silanization reagents and grafted onto the surface. The adsorbent was then molecularly imprinted with dopamine to obtain boric acid modified polyoxometalate molecularly imprinted polymer (Cu@B@PW@MIPs). This adsorbent was used to immobilize HRP, which served as a biological enzyme catalyst from horseradish. The adsorbent was characterized, and its synthetic conditions, experimental conditions, selectivity, reproducibility, and reusability were evaluated. The maximum adsorption amount of HRP under optimized conditions was 159.1 mg g, as determined using high-performance liquid chromatography (HPLC). At pH 7.0, the immobilized enzyme showed a high efficiency of up to 90.0% in removing phenol, after 20 min of reaction with 25 mmol L HO and 0.20 mg mL Cu@B@PW@HRP. Growth tests of aquatic plants confirmed that the adsorbent reduced harm. Gas chromatography-mass spectrometry (GC-MS) tests revealed that the degraded phenol solution contained about fifteen phenol derivatives intermediates. This adsorbent has the potential to become a promising biological enzyme catalyst for dephenolization.
从废水中降解苯酚对于环境保护至关重要。辣根过氧化物酶（HRP）等生物酶在苯酚降解方面显示出巨大的潜力。在这项研究中，我们通过水热法制备了具有杨桃基质形状的空心 CuO/CuO 八面体吸附剂。通过硅烷乳液自组装对吸附剂表面进行修饰，其中 3-氨苯基硼酸（APBA）和多酸（PW）与硅烷化试剂结合并接枝到表面上。然后，用多巴胺对吸附剂进行分子印迹，得到硼酸改性多酸分子印迹聚合物（Cu@B@PW@MIPs）。该吸附剂用于固定 HRP，HRP 是从辣根中提取的生物酶催化剂。对吸附剂进行了表征，并对其合成条件、实验条件、选择性、重现性和可重复性进行了评估。在优化条件下，使用高效液相色谱（HPLC）测定 HRP 的最大吸附量为 159.1mg/g。在 pH 7.0 时，固定化酶在 25mmol/L HO 和 0.20mg/mL Cu@B@PW@HRP 反应 20min 后，对苯酚的去除效率高达 90.0%。水生植物生长试验证实，该吸附剂降低了危害。气相色谱-质谱（GC-MS）测试表明，降解后的苯酚溶液中含有约十五种苯酚衍生物中间体。该吸附剂有望成为一种有前途的苯酚脱除生物酶催化剂。
