Murdoch Children's Research Institute, Parkville, Australia.
Department of Paediatrics, The University of Melbourne, Parkville, Australia.
Methods Mol Biol. 2023;2663:775-786. doi: 10.1007/978-1-0716-3175-1_52.
Blood clot formation represents a key component of the coagulation process for preventing excessive hemorrhage. The structural characteristics of blood clots are associated with their strength and susceptibility to fibrinolysis. Scanning electron microscopy is a technique that allows for state-of-the-art image capture of blood clots, providing visualization of topography, fibrin thickness, fibrin network density, and blood cell involvement and morphology. In this chapter, we provide a detailed protocol for characterization of plasma and whole blood clot structure using SEM, covering the spectrum from blood collection, in vitro clot formation, sample preparation for SEM, imaging, and image analysis, specifically focusing on the measurement of fibrin fiber thickness.
血液凝块的形成是防止过度出血的凝血过程的关键组成部分。血栓的结构特征与其强度和对纤维蛋白溶解的敏感性有关。扫描电子显微镜技术是一种能够对血栓进行最先进的图像捕获的技术，可对形貌、纤维蛋白厚度、纤维蛋白网络密度以及血细胞的参与和形态进行可视化。在本章中，我们提供了使用 SEM 对血浆和全血凝块结构进行特征描述的详细方案，涵盖了从血液采集、体外凝块形成、SEM 样品制备、成像和图像分析的范围，特别是重点介绍了纤维蛋白纤维厚度的测量。
