Liebe S, Weber J, Siegmund E, Bendel L, Arendt R
Dtsch Z Verdau Stoffwechselkr. 1986;46(1):45-52.
A procedure to collect pure pancreatic juice endoscopically is described which allows an instant estimation of the protein content of the secretion by measuring its absorption at 280 nm. By this the kinetics of protein secretion can already be read from a curve during the collecting procedure, thus avoiding a multitude of small fractions otherwise necessary. Timing and size of the samples are adjusted individually according to the course of protein secretion and not to a rigid regimen. The method described is especially apt to investigations of pancreatic proteins and substances the secretion of which parallels that of protein; e. g. trypsinogen, calcium and zinc, however not sodium and potassium.
本文描述了一种通过内镜收集纯胰液的方法，该方法可通过测量其在280nm处的吸光度来即时估算分泌物中的蛋白质含量。通过这种方法，在收集过程中即可从曲线上读取蛋白质分泌的动力学，从而避免了否则需要的大量小份样本。样本的时间和大小根据蛋白质分泌过程单独调整，而不是遵循严格的方案。所描述的方法特别适用于对胰腺蛋白质以及其分泌与蛋白质分泌平行的物质的研究；例如胰蛋白酶原、钙和锌，但不适用于钠和钾。
