Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, Douala, Cameroon.
Laboratory of Biology and Physiology of Animal Organisms, Department of Biology of Animal Organisms, Faculty of Science, The University of Douala Cameroon, Douala, Cameroon.
J Complement Integr Med. 2023 May 22;21(1):1-13. doi: 10.1515/jcim-2023-0017. eCollection 2024 Mar 1.
(, Bignoniaceae) is mainly found in tropical/neotropical regions of America, Africa and Asia. The plant's leaves, stems or roots are used to treat anaemia, bloody diarrhoea, parasitic and microbial infections. This study evaluates anti-inflammatory properties () of and their curative effects on paclitaxel-induced intestinal toxicity ().
The anti-inflammatory potential of was tested over cytokines (TNF-alpha, IL-6, IL-1β, IL-10), reactive oxygen species (ROS) and enzymes (cyclooxygenase and 5-lipoxygenase). While , intestinal toxicity was induced for 10 days by oral administration of paclitaxel (3 mg/kg, 0.05 mL). Animals in each group were further treated with aqueous (300 mg/kg) and ethanolic (300 mg/kg) leaves extracts of during 7 days and clinical symptoms were recorded, hematological, biochemical and histological analysis were subsequently performed.
, aqueous (250 μg/mL) and ethanolic (250 μg/mL) extracts of inhibited the activities of cyclooxygenase 1 (56.67 % and 69.38 %), cyclooxygenase 2 (50.67 % and 62.81 %) and 5-lipoxygenase (77.33 % and 86.00 %). These extracts inhibited the production of intracellular ROS, extracellular ROS and cell proliferation with maximum IC of 30.83 μg/mL, 38.67 μg/mL and 19.05 μg/mL respectively for the aqueous extract, then 25.46 μg/mL, 27.64 μg/mL and 7.34 μg/mL respectively for the ethanolic extract. The extracts also inhibited the production of proinflammatory cytokines (TNFα, IL-1β and IL-6) and stimulated the production of anti-inflammatory cytokines (IL-10). , after administration of paclitaxel, the aqueous and ethanolic extracts of significantly reduced the weight loss, the diarrheal stools and the mass/length intestines ratio of the treated animals compared to the animals of the negative control group. Biochemically, the extracts lead to a significant drop in serum creatinine and alanine aminotransferase levels, followed by a significant increase in alkaline phosphatase. In addition to bringing the haematological parameters back to normal values after disturbance by paclitaxel, the extracts caused tissue regeneration in the treated animals.
, aqueous and ethanolic extracts of showed anti-inflammatory properties (inhibition of COX1, COX2, 5-LOX activities, inhibition of ROS production and cell proliferation); , the same extracts showed curative properties against intestinal toxicity caused by paclitaxel.
（紫葳科）主要分布在美洲、非洲和亚洲的热带/新热带地区。该植物的叶、茎或根可用于治疗贫血、血性腹泻、寄生虫和微生物感染。本研究评估了 （）的抗炎特性及其对紫杉醇诱导的肠道毒性（）的治疗效果。
通过细胞因子（TNF-α、IL-6、IL-1β、IL-10）、活性氧（ROS）和酶（环加氧酶和 5-脂加氧酶）来测试 的抗炎潜力。同时，用紫杉醇（3mg/kg，0.05mL）口服给药 10 天诱导肠道毒性。每组动物进一步用 （300mg/kg）的水提物和 （300mg/kg）的醇提物治疗 7 天，并记录临床症状，随后进行血液学、生化和组织学分析。
（）水提物（250μg/mL）和醇提物（250μg/mL）抑制环加氧酶 1（56.67%和 69.38%）、环加氧酶 2（50.67%和 62.81%）和 5-脂加氧酶（77.33%和 86.00%）的活性。这些提取物抑制细胞内 ROS、细胞外 ROS 和细胞增殖的产生，水提取物的最大 IC 分别为 30.83μg/mL、38.67μg/mL 和 19.05μg/mL，醇提取物的最大 IC 分别为 25.46μg/mL、27.64μg/mL 和 7.34μg/mL。提取物还抑制促炎细胞因子（TNFα、IL-1β 和 IL-6）的产生，并刺激抗炎细胞因子（IL-10）的产生。（），在给予紫杉醇后，与阴性对照组动物相比，（）的水提物和醇提物显著降低了处理动物的体重减轻、腹泻粪便和肠质量/长度比。在生化方面，提取物导致血清肌酐和丙氨酸氨基转移酶水平显著下降，随后碱性磷酸酶水平显著升高。除了使血液学参数在紫杉醇干扰后恢复正常值外，提取物还导致处理动物的组织再生。
（）水提物和醇提物具有抗炎特性（抑制 COX1、COX2、5-LOX 活性、抑制 ROS 产生和细胞增殖）；（），相同的提取物对紫杉醇引起的肠道毒性具有治疗作用。
